Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly; Paul J. Conroy; Stephen Hearty; Tia E. Keyes; Richard O'Kennedy; Robert J. Forster; Lynn Dennany

doi:10.1039/c5ra08450d

Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly, Paul J. Conroy, Stephen Hearty, Tia E. Keyes, Richard O'Kennedy, Robert J. Forster, Lynn Dennany

Department of Chemical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL^-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

Original language	English
Pages (from-to)	67874-67877
Number of pages	4
Journal	RSC Advances
Volume	5
Issue number	83
DOIs	https://doi.org/10.1039/c5ra08450d
Publication status	Published - 31 Jul 2015

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title = "Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection",

abstract = "This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.",

author = "O'Reilly, {Emmet J.} and Conroy, {Paul J.} and Stephen Hearty and Keyes, {Tia E.} and Richard O'Kennedy and Forster, {Robert J.} and Lynn Dennany",

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AU - O'Reilly, Emmet J.

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AU - Hearty, Stephen

AU - Keyes, Tia E.

AU - O'Kennedy, Richard

AU - Forster, Robert J.

AU - Dennany, Lynn

PY - 2015/7/31

Y1 - 2015/7/31

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AB - This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

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Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Abstract

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