Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly; Paul J. Conroy; Stephen Hearty; Tia E. Keyes; Richard O'Kennedy; Robert J. Forster; Lynn Dennany

doi:10.1039/c5ra08450d

Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Emmet J. O'Reilly
, Paul J. Conroy
, Stephen Hearty
, Tia E. Keyes
, Richard O'Kennedy
, Robert J. Forster
, Lynn Dennany

Department of Chemical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL^-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

Original language	English
Pages (from-to)	67874-67877
Number of pages	4
Journal	RSC Advances
Volume	5
Issue number	83
DOIs	https://doi.org/10.1039/c5ra08450d
Publication status	Published - 31 Jul 2015

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title = "Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection",

abstract = "This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.",

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AU - Conroy, Paul J.

AU - Hearty, Stephen

AU - Keyes, Tia E.

AU - O'Kennedy, Richard

AU - Forster, Robert J.

AU - Dennany, Lynn

PY - 2015/7/31

Y1 - 2015/7/31

N2 - This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

AB - This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.

UR - https://www.scopus.com/pages/publications/84939161194

U2 - 10.1039/c5ra08450d

DO - 10.1039/c5ra08450d

M3 - Article

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EP - 67877

JO - RSC Advances

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ER -

Electrochemiluminescence platform for the detection of C-reactive proteins: application of recombinant antibody technology to cardiac biomarker detection

Abstract

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