TY - JOUR
T1 - Endoplasmic Reticulum Stress-Regulated Chaperones as a Serum Biomarker Panel for Parkinson’s Disease
AU - Mnich, Katarzyna
AU - Moghaddam, Shirin
AU - Browne, Patrick
AU - Counihan, Timothy
AU - Fitzgerald, Stephen P.
AU - Martin, Kenneth
AU - Richardson, Ciaran
AU - Samali, Afshin
AU - Gorman, Adrienne M.
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2023/3
Y1 - 2023/3
N2 - Examination of post-mortem brain tissues has previously revealed a strong association between Parkinson’s disease (PD) pathophysiology and endoplasmic reticulum (ER) stress. Evidence in the literature regarding the circulation of ER stress-regulated factors released from neurons provides a rationale for investigating ER stress biomarkers in the blood to aid diagnosis of PD. The levels of ER stress-regulated proteins in serum collected from 29 PD patients and 24 non-PD controls were measured using enzyme-linked immunosorbent assays. A panel of four biomarkers, protein disulfide-isomerase A1, protein disulfide-isomerase A3, mesencephalic astrocyte-derived neurotrophic factor, and clusterin, together with age and gender had higher ability (area under the curve 0.64, sensitivity 66%, specificity 57%) and net benefit to discriminate PD patients from the non-PD group compared with other analyzed models. Addition of oligomeric and total α-synuclein to the model did not improve the diagnostic power of the biomarker panel. We provide evidence that ER stress-regulated proteins merit further investigation for their potential as diagnostic biomarkers of PD. Graphical Abstract: [Figure not available: see fulltext.].
AB - Examination of post-mortem brain tissues has previously revealed a strong association between Parkinson’s disease (PD) pathophysiology and endoplasmic reticulum (ER) stress. Evidence in the literature regarding the circulation of ER stress-regulated factors released from neurons provides a rationale for investigating ER stress biomarkers in the blood to aid diagnosis of PD. The levels of ER stress-regulated proteins in serum collected from 29 PD patients and 24 non-PD controls were measured using enzyme-linked immunosorbent assays. A panel of four biomarkers, protein disulfide-isomerase A1, protein disulfide-isomerase A3, mesencephalic astrocyte-derived neurotrophic factor, and clusterin, together with age and gender had higher ability (area under the curve 0.64, sensitivity 66%, specificity 57%) and net benefit to discriminate PD patients from the non-PD group compared with other analyzed models. Addition of oligomeric and total α-synuclein to the model did not improve the diagnostic power of the biomarker panel. We provide evidence that ER stress-regulated proteins merit further investigation for their potential as diagnostic biomarkers of PD. Graphical Abstract: [Figure not available: see fulltext.].
KW - Biomarker
KW - Chaperone
KW - Endoplasmic reticulum (ER) stress
KW - Parkinson’s disease (PD)
KW - Serum
UR - http://www.scopus.com/inward/record.url?scp=85143413583&partnerID=8YFLogxK
U2 - 10.1007/s12035-022-03139-0
DO - 10.1007/s12035-022-03139-0
M3 - Article
C2 - 36478320
AN - SCOPUS:85143413583
SN - 0893-7648
VL - 60
SP - 1476
EP - 1485
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 3
ER -