TY - JOUR
T1 - Enhanced in vivo visualization of the microcirculation by topical application of fructose solution confirmed with correlation mapping optical coherence tomography
AU - Enfield, Joey
AU - McGrath, James
AU - Daly, Susan M.
AU - Leahy, Martin
N1 - Publisher Copyright:
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Changes within the microcirculation can provide an early indication of the onset of a plethora of ailments. Various techniques have thus been developed that enable the study of microcirculatory irregularities. Correlation mapping optical coherence tomography (cmOCT) is a recently proposed technique, which enables mapping of vasculature networks at the capillary level in a noninvasive and noncontact manner. This technique is an extension of conventional optical coherence tomography (OCT) and is therefore likewise limited in the penetration depth of ballistic photons in biological media. Optical clearing has previously been demonstrated to enhance the penetration depth and the imaging capabilities of OCT. In order to enhance the achievable maximum imaging depth, we propose the use of optical clearing in conjunction with the cmOCT technique. We demonstrate in vivo a 13% increase in OCT penetration depth by topical application of a high-concentration fructose solution, thereby enabling the visualization of vessel features at deeper depths within the tissue.
AB - Changes within the microcirculation can provide an early indication of the onset of a plethora of ailments. Various techniques have thus been developed that enable the study of microcirculatory irregularities. Correlation mapping optical coherence tomography (cmOCT) is a recently proposed technique, which enables mapping of vasculature networks at the capillary level in a noninvasive and noncontact manner. This technique is an extension of conventional optical coherence tomography (OCT) and is therefore likewise limited in the penetration depth of ballistic photons in biological media. Optical clearing has previously been demonstrated to enhance the penetration depth and the imaging capabilities of OCT. In order to enhance the achievable maximum imaging depth, we propose the use of optical clearing in conjunction with the cmOCT technique. We demonstrate in vivo a 13% increase in OCT penetration depth by topical application of a high-concentration fructose solution, thereby enabling the visualization of vessel features at deeper depths within the tissue.
KW - biomedical optical imaging
KW - correlation mapping
KW - microcirculation imaging
KW - optical clearing
KW - optical coherence tomography
UR - http://www.scopus.com/inward/record.url?scp=84975457199&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.21.8.081212
DO - 10.1117/1.JBO.21.8.081212
M3 - Article
C2 - 27311423
AN - SCOPUS:84975457199
SN - 1083-3668
VL - 21
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 8
M1 - 7101007
ER -