TY - JOUR
T1 - Enzyme-assisted extraction of anthocyanins and other phenolic compounds from blackcurrant (Ribes nigrum L.) press cake
T2 - From processing to bioactivities
AU - Granato, Daniel
AU - Fidelis, Marina
AU - Haapakoski, Marjo
AU - dos Santos Lima, Amanda
AU - Viil, Janeli
AU - Hellström, Jarkko
AU - Rätsep, Reelika
AU - Kaldmäe, Hedi
AU - Bleive, Uko
AU - Azevedo, Luciana
AU - Marjomäki, Varpu
AU - Zharkovsky, Alexander
AU - Pap, Nora
N1 - Publisher Copyright:
© 2022 The Author(s)
PY - 2022/10/15
Y1 - 2022/10/15
N2 - The effects of commercial enzymes (pectinases, cellulases, beta-1-3-glucanases, and pectin lyases) on the recovery of anthocyanins and polyphenols from blackcurrant press cake were studied considering two solid:solvent ratios (1:10 and 1:4 w/v). β-glucanase enabled the recovery of the highest total phenolic content – 1142 mg/100 g, and the extraction of anthocyanins was similar using all enzymes (∼400 mg/100 g). The use of cellulases and pectinases enhanced the extraction of antioxidants (DPPH − 1080 mg/100 g; CUPRAC – 3697 mg/100 g). The freeze-dried extracts presented antioxidant potential (CUPRAC, DPPH), which was associated with their biological effects in different systems: antiviral activity against both non-enveloped viruses (enterovirus coxsackievirus A-9) and enveloped coronaviruses (HCoV-OC43), and cytotoxicity towards cancer cells (A549 and HCT8). No cytotoxic effects on normal human lung fibroblast (IMR90) were observed, and no anti-inflammatory activity was detected in lipopolysaccharides-treated murine immortalised microglial cells.
AB - The effects of commercial enzymes (pectinases, cellulases, beta-1-3-glucanases, and pectin lyases) on the recovery of anthocyanins and polyphenols from blackcurrant press cake were studied considering two solid:solvent ratios (1:10 and 1:4 w/v). β-glucanase enabled the recovery of the highest total phenolic content – 1142 mg/100 g, and the extraction of anthocyanins was similar using all enzymes (∼400 mg/100 g). The use of cellulases and pectinases enhanced the extraction of antioxidants (DPPH − 1080 mg/100 g; CUPRAC – 3697 mg/100 g). The freeze-dried extracts presented antioxidant potential (CUPRAC, DPPH), which was associated with their biological effects in different systems: antiviral activity against both non-enveloped viruses (enterovirus coxsackievirus A-9) and enveloped coronaviruses (HCoV-OC43), and cytotoxicity towards cancer cells (A549 and HCT8). No cytotoxic effects on normal human lung fibroblast (IMR90) were observed, and no anti-inflammatory activity was detected in lipopolysaccharides-treated murine immortalised microglial cells.
KW - Anti-inflammatory activity
KW - Antioxidant activity
KW - Antiproliferative activity
KW - Antiviral activity
KW - Blackcurrant side-streams
KW - Circular economy
KW - Polyphenols
UR - https://www.scopus.com/pages/publications/85131702130
U2 - 10.1016/j.foodchem.2022.133240
DO - 10.1016/j.foodchem.2022.133240
M3 - Article
C2 - 35617760
AN - SCOPUS:85131702130
SN - 0308-8146
VL - 391
JO - Food Chemistry
JF - Food Chemistry
M1 - 133240
ER -
