TY - JOUR
T1 - Enzyme-assisted release of antioxidant peptides from porphyra dioica conchocelis
AU - Pimentel, Filipa B.
AU - Machado, Marlene
AU - Cermeño, Maria
AU - Kleekayai, Thanyaporn
AU - Machado, Susana
AU - Rego, Andreia M.
AU - Abreu, Maria H.
AU - Alves, Rita C.
AU - Oliveira, Maria Beatriz P.P.
AU - Fitzgerald, Richard J.
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/2
Y1 - 2021/2
N2 - The conchocelis life cycle stage of P. dioica represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve® and Flavourzyme®). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained ~25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients.
AB - The conchocelis life cycle stage of P. dioica represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve® and Flavourzyme®). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained ~25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients.
KW - Amino acids
KW - Antioxidant activity
KW - Enzyme-assisted release
KW - Porphyra dioica conchocelis
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85100474006&partnerID=8YFLogxK
U2 - 10.3390/antiox10020249
DO - 10.3390/antiox10020249
M3 - Article
AN - SCOPUS:85100474006
VL - 10
SP - 1
EP - 13
JO - Antioxidants
JF - Antioxidants
IS - 2
M1 - 249
ER -