Extraction and Characterization of Protein Concentrates from Limpets (Patella vulgata) and Peptide Release Following Gastrointestinal Digestion

Gurkan Bilir, Mohammadreza Khalesi, Maria Cermeño, Richard J. Fitzgerald, Deniz Ekinci

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigated the characterization of proteins from the Irish limpet (Patella vulgata) and assessed the in vitro biological activities of hydrolysates obtained following gastrointestinal digestion (INFOGEST) of a limpet protein concentrate (LPC). The physicochemical properties and the digestibility of the LPC were investigated, along with the angiotensin-converting enzyme (ACE) inhibition and antioxidant activities of the LPC-digested samples. All the digested samples examined outperformed the LPC in terms of activity. Peptides were identified using LC-MS/MS after digestion. A total of 38 and 19 peptides were identified in LPC-G and LPC-GI, respectively, using a database search and a de novo approach. Most of the identified peptides had hydrophobic amino acids, which may contribute to their antioxidant and ACE inhibitory activities. The findings of this study showed that LPC has high nutritional quality with good digestibility and could serve as a potential source of antioxidative and ACE inhibitory peptides following gastrointestinal digestion.

Original languageEnglish
Pages (from-to)11212-11223
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume70
Issue number36
DOIs
Publication statusPublished - 14 Sep 2022

Keywords

  • ACE inhibitor
  • PDCAAS
  • Patella vulgata
  • antioxidant
  • digestibility
  • in vitro bioactivity
  • limpet
  • peptide
  • solubility

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