TY - JOUR
T1 - Extraction optimization of bioactive compounds from ora-pro-nobis (Pereskia aculeata Miller) leaves and their in vitro antioxidant and antihemolytic activities
AU - Cruz, Thiago Mendanha
AU - Santos, Jânio Sousa
AU - do Carmo, Mariana Araújo Vieira
AU - Hellström, Jarkko
AU - Pihlava, Juha Matti
AU - Azevedo, Luciana
AU - Granato, Daniel
AU - Marques, Mariza Boscacci
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/11/1
Y1 - 2021/11/1
N2 - Ora-pro-nobis (Pereskia aculeata Miller) is a non-conventional food plant common in Brazil. The objective of this study was to optimize the extraction of bioactive phenolic compounds from ora-pro-nobis leaves by employing solvent mixtures. Ten extracts were obtained with water, ethanol, acetone, and their binary and ternary mixtures, evaluating the chemical composition, antioxidant activity and bioactivities in vitro. The response surface methodology was applied to model the results and calculate the optimal solvent composition, which is 60% water, 40% ethanol and 0% acetone. The optimized extract is rich in phenolic compounds (64 mg GAE/g) and proteins (823 mg/g) and presents antioxidant activity (in intracellular media as well) and inhibits lipid peroxidation (32%) along with hypotonic hemolysis (H50 = 0.339%), it does not present toxicity in vitro against cancer and normal cells. This is the first report of chicoric, caffeoyl-hexaric and coumaroyl-hexaric acids and some glycosylate derivatives of flavonols in ora-pro-nobis leaves.
AB - Ora-pro-nobis (Pereskia aculeata Miller) is a non-conventional food plant common in Brazil. The objective of this study was to optimize the extraction of bioactive phenolic compounds from ora-pro-nobis leaves by employing solvent mixtures. Ten extracts were obtained with water, ethanol, acetone, and their binary and ternary mixtures, evaluating the chemical composition, antioxidant activity and bioactivities in vitro. The response surface methodology was applied to model the results and calculate the optimal solvent composition, which is 60% water, 40% ethanol and 0% acetone. The optimized extract is rich in phenolic compounds (64 mg GAE/g) and proteins (823 mg/g) and presents antioxidant activity (in intracellular media as well) and inhibits lipid peroxidation (32%) along with hypotonic hemolysis (H50 = 0.339%), it does not present toxicity in vitro against cancer and normal cells. This is the first report of chicoric, caffeoyl-hexaric and coumaroyl-hexaric acids and some glycosylate derivatives of flavonols in ora-pro-nobis leaves.
KW - Aldaric acid esters of phenolic acids
KW - Flavonols
KW - Hemolysis
KW - Non-conventional food plant
KW - Reactive oxygen species
KW - Response surface methodology
UR - http://www.scopus.com/inward/record.url?scp=85108741902&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2021.130078
DO - 10.1016/j.foodchem.2021.130078
M3 - Article
C2 - 34023692
AN - SCOPUS:85108741902
SN - 0308-8146
VL - 361
SP - 130078
JO - Food Chemistry
JF - Food Chemistry
M1 - 130078
ER -