TY - JOUR
T1 - Fabricating Covalent Organic Framework Capsules with Commodious Microenvironment for Enzymes
AU - Li, Mingmin
AU - Qiao, Shan
AU - Zheng, Yunlong
AU - Andaloussi, Yassin H.
AU - Li, Xia
AU - Zhang, Zhenjie
AU - Li, Ang
AU - Cheng, Peng
AU - Ma, Shengqian
AU - Chen, Yao
N1 - Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/4/8
Y1 - 2020/4/8
N2 - Enzyme immobilization has been demonstrated to be a favorable protocol to promote industrialization of biomacromolecules. Despite tremendous efforts to develop new strategies and materials to realize this process, maintaining enzyme activity is still a formidable challenge. Herein we created a sacrificial templating method, using metal-organic frameworks (MOFs) as sacrificial templates to construct hollow covalent organic framework (COF) capsules for enzyme encapsulation. This strategy can provide a capacious microenvironment to unleash enzyme molecules. The improved conformational freedom of enzymes, enhanced mass transfer, and protective effect against the external environment ultimately boosted the enzymatic activities. We also found that this strategy possesses high versatility that is suitable for diverse biomacromolecules, MOF templates, and COF capsules. Moreover, the dimensions, pore sizes, and shell thickness of COF capsules can be conveniently tuned, allowing for customizing bioreactors for specific functions. For example, coencapsulation of different enzymes with synergistic functions were successfully demonstrated using this bioreactor platform. This study not only opens up a new avenue to overcome the present limitations of enzymatic immobilization in porous matrixes but also provides new opportunities for construction of biomicrodevices or artificial organelles based on crystalline porous materials.
AB - Enzyme immobilization has been demonstrated to be a favorable protocol to promote industrialization of biomacromolecules. Despite tremendous efforts to develop new strategies and materials to realize this process, maintaining enzyme activity is still a formidable challenge. Herein we created a sacrificial templating method, using metal-organic frameworks (MOFs) as sacrificial templates to construct hollow covalent organic framework (COF) capsules for enzyme encapsulation. This strategy can provide a capacious microenvironment to unleash enzyme molecules. The improved conformational freedom of enzymes, enhanced mass transfer, and protective effect against the external environment ultimately boosted the enzymatic activities. We also found that this strategy possesses high versatility that is suitable for diverse biomacromolecules, MOF templates, and COF capsules. Moreover, the dimensions, pore sizes, and shell thickness of COF capsules can be conveniently tuned, allowing for customizing bioreactors for specific functions. For example, coencapsulation of different enzymes with synergistic functions were successfully demonstrated using this bioreactor platform. This study not only opens up a new avenue to overcome the present limitations of enzymatic immobilization in porous matrixes but also provides new opportunities for construction of biomicrodevices or artificial organelles based on crystalline porous materials.
UR - http://www.scopus.com/inward/record.url?scp=85083822504&partnerID=8YFLogxK
U2 - 10.1021/jacs.0c00285
DO - 10.1021/jacs.0c00285
M3 - Article
C2 - 32197569
AN - SCOPUS:85083822504
SN - 0002-7863
VL - 142
SP - 6675
EP - 6681
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 14
ER -