Gene Expression Analysis of Mouse Embryonic Stem Cells Following Levitation in an Ultrasound Standing Wave Trap

Despina Bazou, Roisin Kearney, Fiona Mansergh, Celine Bourdon, Jane Farrar, Michael Wride

Research output: Contribution to journalArticlepeer-review

Abstract

In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08-0.85 MPa) and times (5-60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine.

Original languageEnglish
Pages (from-to)321-330
Number of pages10
JournalUltrasound in Medicine and Biology
Volume37
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Keywords

  • Cell manipulation
  • Differentiation
  • Embryoid bodies
  • Embryonic stem cells
  • Gene expression
  • Microenvironment
  • Neural
  • Pluripotency
  • Ultrasound

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