Human insulin and desamido human insulin isotherms in ethanol-water reversed phase systems

Y. Zhou; M. Ottens; E. Hansen; L. A.M. Van Der Wielen

doi:10.1016/j.chroma.2004.10.089

Human insulin and desamido human insulin isotherms in ethanol-water reversed phase systems

Y. Zhou, M. Ottens, E. Hansen, L. A.M. Van Der Wielen

Research output: Contribution to journal › Article › peer-review

Abstract

The multi-component isotherms for human insulin (HI) and desamido human insulin (dHI) over reversed phase packing (C ₁₈) and with 29.8% (w/w) ethanol-water as mobile phase have been determined experimentally. The isotherms of HI in ethanol-water differ from those obtained with the more commonly applied methanol-water and acetonitrile-water mobile phase, as described in this paper. The isotherm exhibits anti Langmuirian behavior and can be very well modeled by an anti Langmuir isotherm presented in this paper. The HI and dHI anti Langmuir isotherm are determined as: qHI = 8.4C _HI + 3C _HIC _dHI/1-0.05C _HI - 0.14C _dHI + 0.04C _HIC _dHI and q _dHI = 11.4C _dHI + 2C _HIC _dHI/1-0.05C _HI - 0.14C _dHI + 0.04C _HIC _dHI

Original language	English
Pages (from-to)	141-148
Number of pages	8
Journal	Journal of Chromatography A
Volume	1061
Issue number	2
DOIs	https://doi.org/10.1016/j.chroma.2004.10.089
Publication status	Published - 24 Dec 2004
Externally published	Yes

Keywords

Adsorption isotherms
Anti-Langmuir isotherm
Desamido human insulin
Human insulin
Multimerization
Reversed phase chromatography

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10.1016/j.chroma.2004.10.089

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title = "Human insulin and desamido human insulin isotherms in ethanol-water reversed phase systems",

abstract = "The multi-component isotherms for human insulin (HI) and desamido human insulin (dHI) over reversed phase packing (C 18) and with 29.8% (w/w) ethanol-water as mobile phase have been determined experimentally. The isotherms of HI in ethanol-water differ from those obtained with the more commonly applied methanol-water and acetonitrile-water mobile phase, as described in this paper. The isotherm exhibits anti Langmuirian behavior and can be very well modeled by an anti Langmuir isotherm presented in this paper. The HI and dHI anti Langmuir isotherm are determined as: qHI = 8.4C HI + 3C HIC dHI/1-0.05C HI - 0.14C dHI + 0.04C HIC dHI and q dHI = 11.4C dHI + 2C HIC dHI/1-0.05C HI - 0.14C dHI + 0.04C HIC dHI",

keywords = "Adsorption isotherms, Anti-Langmuir isotherm, Desamido human insulin, Human insulin, Multimerization, Reversed phase chromatography",

author = "Y. Zhou and M. Ottens and E. Hansen and {Van Der Wielen}, {L. A.M.}",

year = "2004",

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doi = "10.1016/j.chroma.2004.10.089",

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AU - Zhou, Y.

AU - Ottens, M.

AU - Hansen, E.

AU - Van Der Wielen, L. A.M.

PY - 2004/12/24

Y1 - 2004/12/24

N2 - The multi-component isotherms for human insulin (HI) and desamido human insulin (dHI) over reversed phase packing (C 18) and with 29.8% (w/w) ethanol-water as mobile phase have been determined experimentally. The isotherms of HI in ethanol-water differ from those obtained with the more commonly applied methanol-water and acetonitrile-water mobile phase, as described in this paper. The isotherm exhibits anti Langmuirian behavior and can be very well modeled by an anti Langmuir isotherm presented in this paper. The HI and dHI anti Langmuir isotherm are determined as: qHI = 8.4C HI + 3C HIC dHI/1-0.05C HI - 0.14C dHI + 0.04C HIC dHI and q dHI = 11.4C dHI + 2C HIC dHI/1-0.05C HI - 0.14C dHI + 0.04C HIC dHI

AB - The multi-component isotherms for human insulin (HI) and desamido human insulin (dHI) over reversed phase packing (C 18) and with 29.8% (w/w) ethanol-water as mobile phase have been determined experimentally. The isotherms of HI in ethanol-water differ from those obtained with the more commonly applied methanol-water and acetonitrile-water mobile phase, as described in this paper. The isotherm exhibits anti Langmuirian behavior and can be very well modeled by an anti Langmuir isotherm presented in this paper. The HI and dHI anti Langmuir isotherm are determined as: qHI = 8.4C HI + 3C HIC dHI/1-0.05C HI - 0.14C dHI + 0.04C HIC dHI and q dHI = 11.4C dHI + 2C HIC dHI/1-0.05C HI - 0.14C dHI + 0.04C HIC dHI

KW - Adsorption isotherms

KW - Anti-Langmuir isotherm

KW - Desamido human insulin

KW - Human insulin

KW - Multimerization

KW - Reversed phase chromatography

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IS - 2

ER -

Human insulin and desamido human insulin isotherms in ethanol-water reversed phase systems

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Keywords

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