Hydrogen peroxide generation by the Weissberger biogenic oxidative system during hyaluronan degradation

Katarina Valachová; Dominika Topol'ská; Raniero Mendichi; Maurice N. Collins; Vlasta Sasinková; Ladislav Šoltés

doi:10.1016/j.carbpol.2016.04.063

Hydrogen peroxide generation by the Weissberger biogenic oxidative system during hyaluronan degradation

Katarina Valachová, Dominika Topol'ská, Raniero Mendichi, Maurice N. Collins, Vlasta Sasinková, Ladislav Šoltés

Research output: Contribution to journal › Article › peer-review

Abstract

By applying the enzyme catalase, our study on hyaluronan degradation confirms the generation of hydrogen peroxide using the Weissberger biogenic oxidative system (WBOS), which is composed of ascorbate and cupric ions. Dynamic viscosities of hyaluronan (HA) solutions influenced by WBOS in the absence and presence of catalase were analysed by rotational viscometry. Molar masses of HAs were determined by size-exclusion chromatography with multi-angle laser-light scattering. Our results show that catalase dose-dependently inhibited the degradation of HA macromolecules, which presumably confirms the generation of H₂O₂ in the reaction system. This has implications in range of biomedical applications such as arthritic joint treatment, tissue engineering, ocular and cosmetic surgery.

Original language	English
Pages (from-to)	189-193
Number of pages	5
Journal	Carbohydrate Polymers
Volume	148
DOIs	https://doi.org/10.1016/j.carbpol.2016.04.063
Publication status	Published - 5 Sep 2016

Keywords

Catalase
Glycosaminoglycans
Reactive oxygen species

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10.1016/j.carbpol.2016.04.063

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title = "Hydrogen peroxide generation by the Weissberger biogenic oxidative system during hyaluronan degradation",

abstract = "By applying the enzyme catalase, our study on hyaluronan degradation confirms the generation of hydrogen peroxide using the Weissberger biogenic oxidative system (WBOS), which is composed of ascorbate and cupric ions. Dynamic viscosities of hyaluronan (HA) solutions influenced by WBOS in the absence and presence of catalase were analysed by rotational viscometry. Molar masses of HAs were determined by size-exclusion chromatography with multi-angle laser-light scattering. Our results show that catalase dose-dependently inhibited the degradation of HA macromolecules, which presumably confirms the generation of H2O2 in the reaction system. This has implications in range of biomedical applications such as arthritic joint treatment, tissue engineering, ocular and cosmetic surgery.",

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T1 - Hydrogen peroxide generation by the Weissberger biogenic oxidative system during hyaluronan degradation

AU - Valachová, Katarina

AU - Topol'ská, Dominika

AU - Mendichi, Raniero

AU - Collins, Maurice N.

AU - Sasinková, Vlasta

AU - Šoltés, Ladislav

PY - 2016/9/5

Y1 - 2016/9/5

N2 - By applying the enzyme catalase, our study on hyaluronan degradation confirms the generation of hydrogen peroxide using the Weissberger biogenic oxidative system (WBOS), which is composed of ascorbate and cupric ions. Dynamic viscosities of hyaluronan (HA) solutions influenced by WBOS in the absence and presence of catalase were analysed by rotational viscometry. Molar masses of HAs were determined by size-exclusion chromatography with multi-angle laser-light scattering. Our results show that catalase dose-dependently inhibited the degradation of HA macromolecules, which presumably confirms the generation of H2O2 in the reaction system. This has implications in range of biomedical applications such as arthritic joint treatment, tissue engineering, ocular and cosmetic surgery.

AB - By applying the enzyme catalase, our study on hyaluronan degradation confirms the generation of hydrogen peroxide using the Weissberger biogenic oxidative system (WBOS), which is composed of ascorbate and cupric ions. Dynamic viscosities of hyaluronan (HA) solutions influenced by WBOS in the absence and presence of catalase were analysed by rotational viscometry. Molar masses of HAs were determined by size-exclusion chromatography with multi-angle laser-light scattering. Our results show that catalase dose-dependently inhibited the degradation of HA macromolecules, which presumably confirms the generation of H2O2 in the reaction system. This has implications in range of biomedical applications such as arthritic joint treatment, tissue engineering, ocular and cosmetic surgery.

KW - Catalase

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KW - Reactive oxygen species

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Hydrogen peroxide generation by the Weissberger biogenic oxidative system during hyaluronan degradation

Abstract

Keywords

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