TY - JOUR
T1 - Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity
AU - Cermeño, Maria
AU - Bascón, Carmen
AU - Amigo-Benavent, Miryam
AU - Felix, Manuel
AU - FitzGerald, Richard J.
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/5
Y1 - 2022/5
N2 - Silkworm (Bombyx mori) pupae is a by-product from the silk industry which is rich in protein. Hydrolysates from silkworm pupae generated using Alcalase®, Prolyve®, Flavourzyme® and Brewers Clarex® proteolytic preparations were characterised. The antioxidant activity of the hydrolysates was investigated using in vitro antioxidant assays and an in situ assay for reactive oxygen species (ROS) reduction using hepatic HepG2. Overall, Alcalase and Prolyve hydrolysates had highest scavenging activities, however, Flavourzyme and Brewers Clarex hydrolysates had enhanced ferric reducing antioxidant power (FRAP) activity compared to the other samples. Furthermore, the Flavourzyme hydrolysate significantly reduced ROS by 40% compared to untreated control HepG2 cells. Peptides identified by LC-MS/MS were synthetised and then tested for their in vitro and in situ antioxidant activity. Peptides SWFVTPF and NDVLFF showed highest antioxidant activity (ROS reduction, superoxide dismutase (SOD) expression and glutathione (GSH) production activity) in HepG2 cells, and therefore may have potential as natural antioxidants.
AB - Silkworm (Bombyx mori) pupae is a by-product from the silk industry which is rich in protein. Hydrolysates from silkworm pupae generated using Alcalase®, Prolyve®, Flavourzyme® and Brewers Clarex® proteolytic preparations were characterised. The antioxidant activity of the hydrolysates was investigated using in vitro antioxidant assays and an in situ assay for reactive oxygen species (ROS) reduction using hepatic HepG2. Overall, Alcalase and Prolyve hydrolysates had highest scavenging activities, however, Flavourzyme and Brewers Clarex hydrolysates had enhanced ferric reducing antioxidant power (FRAP) activity compared to the other samples. Furthermore, the Flavourzyme hydrolysate significantly reduced ROS by 40% compared to untreated control HepG2 cells. Peptides identified by LC-MS/MS were synthetised and then tested for their in vitro and in situ antioxidant activity. Peptides SWFVTPF and NDVLFF showed highest antioxidant activity (ROS reduction, superoxide dismutase (SOD) expression and glutathione (GSH) production activity) in HepG2 cells, and therefore may have potential as natural antioxidants.
KW - Antioxidant
KW - Bioactive
KW - Bombyx mori
KW - Peptide
KW - Silkworm
UR - http://www.scopus.com/inward/record.url?scp=85127359890&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2022.105052
DO - 10.1016/j.jff.2022.105052
M3 - Article
AN - SCOPUS:85127359890
SN - 1756-4646
VL - 92
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 105052
ER -