Immunohistochemical demonstration of the Epstein–Barr virus‐encoded latent membrane protein in paraffin sections of Hodgkin's disease

Paul G. Murray; Lawrence S. Young; Martin Rowe; John Crocker

doi:10.1002/path.1711660102

Immunohistochemical demonstration of the Epstein–Barr virus‐encoded latent membrane protein in paraffin sections of Hodgkin's disease

Paul G. Murray, Lawrence S. Young, Martin Rowe, John Crocker

Research output: Contribution to journal › Article › peer-review

Abstract

Paraffin sections from 46 cases of Hodgkin's disease were examined for the presence of the Epstein‐Barr virus (EBV)‐encoded latent membrane protein (LMP) using a sensitive (double layer alkaline phosphatase–anti‐alkaline phosphatase) immunohistochemical method. LMP was detected in 22 cases, the majority of positive cases being of nodular sclerosis (12/24), mixed cellularity (6/7), and lymphocyte depletion (3/3) subtypes. Only one of 12 cases of lymphocyte predominant disease was positive. In all cases, reactivity was confined to Hodgkin's and Reed‐Sternberg cells. These results provide further evidence for an association between EBV and Hodgkin's disease and indicate that LMP may be readily detected in archival material.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	The Journal of Pathology
Volume	166
Issue number	1
DOIs	https://doi.org/10.1002/path.1711660102
Publication status	Published - Jan 1992
Externally published	Yes

Keywords

Epstein‐Barr virus
Hodgkin's disease
Latent membrane protein

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10.1002/path.1711660102

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abstract = "Paraffin sections from 46 cases of Hodgkin's disease were examined for the presence of the Epstein‐Barr virus (EBV)‐encoded latent membrane protein (LMP) using a sensitive (double layer alkaline phosphatase–anti‐alkaline phosphatase) immunohistochemical method. LMP was detected in 22 cases, the majority of positive cases being of nodular sclerosis (12/24), mixed cellularity (6/7), and lymphocyte depletion (3/3) subtypes. Only one of 12 cases of lymphocyte predominant disease was positive. In all cases, reactivity was confined to Hodgkin's and Reed‐Sternberg cells. These results provide further evidence for an association between EBV and Hodgkin's disease and indicate that LMP may be readily detected in archival material.",

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AU - Murray, Paul G.

AU - Young, Lawrence S.

AU - Rowe, Martin

AU - Crocker, John

PY - 1992/1

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N2 - Paraffin sections from 46 cases of Hodgkin's disease were examined for the presence of the Epstein‐Barr virus (EBV)‐encoded latent membrane protein (LMP) using a sensitive (double layer alkaline phosphatase–anti‐alkaline phosphatase) immunohistochemical method. LMP was detected in 22 cases, the majority of positive cases being of nodular sclerosis (12/24), mixed cellularity (6/7), and lymphocyte depletion (3/3) subtypes. Only one of 12 cases of lymphocyte predominant disease was positive. In all cases, reactivity was confined to Hodgkin's and Reed‐Sternberg cells. These results provide further evidence for an association between EBV and Hodgkin's disease and indicate that LMP may be readily detected in archival material.

AB - Paraffin sections from 46 cases of Hodgkin's disease were examined for the presence of the Epstein‐Barr virus (EBV)‐encoded latent membrane protein (LMP) using a sensitive (double layer alkaline phosphatase–anti‐alkaline phosphatase) immunohistochemical method. LMP was detected in 22 cases, the majority of positive cases being of nodular sclerosis (12/24), mixed cellularity (6/7), and lymphocyte depletion (3/3) subtypes. Only one of 12 cases of lymphocyte predominant disease was positive. In all cases, reactivity was confined to Hodgkin's and Reed‐Sternberg cells. These results provide further evidence for an association between EBV and Hodgkin's disease and indicate that LMP may be readily detected in archival material.

KW - Epstein‐Barr virus

KW - Hodgkin's disease

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Immunohistochemical demonstration of the Epstein–Barr virus‐encoded latent membrane protein in paraffin sections of Hodgkin's disease

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