TY - JOUR
T1 - Impact of alkaline solution, carbohydrase and biomass:solvent on extraction efficiency, and protein and amino acid profiles of Palmaria palmata protein extracts
AU - Idowu, Anthony Temitope
AU - Amigo-Benavent, Miryam
AU - FitzGerald, Richard J.
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/12
Y1 - 2024/12
N2 - The impact of using different alkaline solutions, carbohydrases and changing the biomass to solvent volume ratio (biomass:solvent) on protein content, protein and biomass yield recovery in protein enriched extracts from Palmaria palmata (red seaweed) was studied. Protein extraction with alkaline solutions, i.e., NaOH, KOH, Ca(OH)2 and Na2CO3 (at 0.12 M) along with (and without) carbohydrases B, BD and L (containing xylanase, a mixture of xylanase and cellulase, and a combination of cellulase, xylanase and polygalacturonase activities, respectively) was assessed. Principal component analysis was used to assess correlations (if any) between the different alkaline solvents, carbohydrases and changing biomass:solvent on extraction efficiency. The highest protein content (56.64 ± 0.43 % (w/w)) was obtained using KOH assisted extraction with carbohydrase BD. The highest protein and biomass yield recoveries (70.65 ± 1.38 and 21.99 ± 0.43 % (w/w), respectively) were obtained on extraction using NaOH assisted with carbohydrases BD. Electrophoretic, amino acid profile and amino acid score analyses displayed differences depending on the alkaline solvent used for protein extraction. Appropriate selection of the alkaline solvent, carbohydrase and biomass:volume combination during protein extraction can contribute to the production of compositionally enhanced high-quality protein concentrates from P. palmata.
AB - The impact of using different alkaline solutions, carbohydrases and changing the biomass to solvent volume ratio (biomass:solvent) on protein content, protein and biomass yield recovery in protein enriched extracts from Palmaria palmata (red seaweed) was studied. Protein extraction with alkaline solutions, i.e., NaOH, KOH, Ca(OH)2 and Na2CO3 (at 0.12 M) along with (and without) carbohydrases B, BD and L (containing xylanase, a mixture of xylanase and cellulase, and a combination of cellulase, xylanase and polygalacturonase activities, respectively) was assessed. Principal component analysis was used to assess correlations (if any) between the different alkaline solvents, carbohydrases and changing biomass:solvent on extraction efficiency. The highest protein content (56.64 ± 0.43 % (w/w)) was obtained using KOH assisted extraction with carbohydrase BD. The highest protein and biomass yield recoveries (70.65 ± 1.38 and 21.99 ± 0.43 % (w/w), respectively) were obtained on extraction using NaOH assisted with carbohydrases BD. Electrophoretic, amino acid profile and amino acid score analyses displayed differences depending on the alkaline solvent used for protein extraction. Appropriate selection of the alkaline solvent, carbohydrase and biomass:volume combination during protein extraction can contribute to the production of compositionally enhanced high-quality protein concentrates from P. palmata.
KW - Amino acid
KW - Cationic effect
KW - Extraction
KW - Palmaria palmata
KW - Recovery
KW - Red seaweed
UR - http://www.scopus.com/inward/record.url?scp=85205311163&partnerID=8YFLogxK
U2 - 10.1016/j.fbp.2024.09.017
DO - 10.1016/j.fbp.2024.09.017
M3 - Article
AN - SCOPUS:85205311163
SN - 0960-3085
VL - 148
SP - 285
EP - 297
JO - Food and Bioproducts Processing
JF - Food and Bioproducts Processing
ER -