TY - JOUR
T1 - In Situ Ionically Cross-Linkable Benzaldehyde-Modified Alginate and Its Bilayer Structure as a Tissue Sealant for Dural Closure
AU - Yang, I-Hsuan
AU - Inagaki, Natsuko F.
AU - Chandel, Arvind K. Singh
AU - Matsumoto, Mifuyu
AU - Yamada, Mai
AU - Matsumiya, Kazuki
AU - Dai, Yizhou
AU - Iwabuchi, Takaya
AU - Onishi, Kotaro
AU - Fukushima, Sonoko
AU - Ohta, Seiichi
AU - Mizushima, Akihiro
AU - Isaji, Mitsuko
AU - Shimizu, Satoshi
AU - Takahata, Masahiko
AU - Iwasaki, Norimasa
AU - Ito, Taichi
N1 - Publisher Copyright:
© 2025 American Chemical Society
PY - 2025/9/8
Y1 - 2025/9/8
N2 - Effective tissue adhesives are vital for surgical applications. We synthesized p-aminobenzaldehyde-modified alginate (AL-ABA) with controlled degrees of substitution (DS, 3.8–13.7%) and cross-linked it ionically using CaCl2. AL-ABA solutions (3 w/v %) gelled rapidly (3–50 s), similar to unmodified alginate. Increasing DS reduced hydrogel stiffness but enhanced dura adhesiveness via Schiff’s base formation with tissue amines, reaching 32 kPa at 9% DS. To optimize both adhesion and mechanical integrity, bilayer hydrogels were developed using an AL-ABA primer and AL cover layer, seamlessly bonded by ionic cross-linking. These AL-ABA/AL hydrogels showed strong dural adhesion and high burst pressure resistance (up to 406 mmHg). AL-ABA retained a low cytotoxicity and hemocompatibility. Ionically cross-linked AL-ABA and its bilayer configuration offer promising potential as biocompatible and efficient tissue sealants.
AB - Effective tissue adhesives are vital for surgical applications. We synthesized p-aminobenzaldehyde-modified alginate (AL-ABA) with controlled degrees of substitution (DS, 3.8–13.7%) and cross-linked it ionically using CaCl2. AL-ABA solutions (3 w/v %) gelled rapidly (3–50 s), similar to unmodified alginate. Increasing DS reduced hydrogel stiffness but enhanced dura adhesiveness via Schiff’s base formation with tissue amines, reaching 32 kPa at 9% DS. To optimize both adhesion and mechanical integrity, bilayer hydrogels were developed using an AL-ABA primer and AL cover layer, seamlessly bonded by ionic cross-linking. These AL-ABA/AL hydrogels showed strong dural adhesion and high burst pressure resistance (up to 406 mmHg). AL-ABA retained a low cytotoxicity and hemocompatibility. Ionically cross-linked AL-ABA and its bilayer configuration offer promising potential as biocompatible and efficient tissue sealants.
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pureapplicaion&SrcAuth=WosAPI&KeyUT=WOS:001555939900001&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1021/acs.biomac.5c00748
DO - 10.1021/acs.biomac.5c00748
M3 - Article
C2 - 40851477
SN - 1525-7797
VL - 26
SP - 5888
EP - 5902
JO - Biomacromolecules
JF - Biomacromolecules
IS - 9
ER -