TY - JOUR
T1 - Influence of tiopronin, captopril and levamisole therapeutics on the oxidative degradation of hyaluronan
AU - Valachová, Katarína
AU - Baňasová, Mária
AU - Topo̘ská, Dominika
AU - Sasinková, Vlasta
AU - Juránek, Ivo
AU - Collins, Maurice N.
AU - Šoltés, Ladislav
N1 - Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.
PY - 2015/12/10
Y1 - 2015/12/10
N2 - The ability to protect hyaluronic acid (HA) from oxidative degradation by cupric ions and ascorbate (production of KOH and peroxy-type radicals) during acute phase joint inflammation has been investigated using the following drugs: tiopronin, captopril, and levamisole. Radical scavenging activity, i.e. the propensity for donation of electrons was assessed for the drugs by ABTS and DPPH assays. The kinetics of HA degradation have been measured in the presence of each drug using rotational viscometry. The results of ABTS and DPPH assays show the highest radical scavenging activity for captopril, followed by tiopronin. For levamisole, no effect was observed. Captopril and tiopronin prevented HA degradation induced by OH radicals in a similar manner, while tiopronin was more effective in scavenging peroxy-type radicals. On the other hand, levamisole was shown to be a pro-oxidant. Recovered HA fragments were characterized using FT-IR analysis, the incorporation of a sulphur atom from captopril and tiopronin but not from levamisole into the HA molecule was demonstrated.
AB - The ability to protect hyaluronic acid (HA) from oxidative degradation by cupric ions and ascorbate (production of KOH and peroxy-type radicals) during acute phase joint inflammation has been investigated using the following drugs: tiopronin, captopril, and levamisole. Radical scavenging activity, i.e. the propensity for donation of electrons was assessed for the drugs by ABTS and DPPH assays. The kinetics of HA degradation have been measured in the presence of each drug using rotational viscometry. The results of ABTS and DPPH assays show the highest radical scavenging activity for captopril, followed by tiopronin. For levamisole, no effect was observed. Captopril and tiopronin prevented HA degradation induced by OH radicals in a similar manner, while tiopronin was more effective in scavenging peroxy-type radicals. On the other hand, levamisole was shown to be a pro-oxidant. Recovered HA fragments were characterized using FT-IR analysis, the incorporation of a sulphur atom from captopril and tiopronin but not from levamisole into the HA molecule was demonstrated.
KW - Abbreviations ABTS 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid
KW - DPPH 2,2-diphenyl-1-picrylhydrazyl
KW - HA hyaluronan
KW - SF synovial fluid
KW - WBOS Weissberger's biogenic oxidative system
UR - http://www.scopus.com/inward/record.url?scp=84940068534&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2015.07.029
DO - 10.1016/j.carbpol.2015.07.029
M3 - Article
C2 - 26428153
AN - SCOPUS:84940068534
SN - 0144-8617
VL - 134
SP - 516
EP - 523
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -