Interfacing Seurat with the R tidy universe

Stefano Mangiola, Maria A. Doyle, Anthony T. Papenfuss

Research output: Contribution to journalArticlepeer-review

Abstract

Motivation: Seurat is one of the most popular software suites for the analysis of single-cell RNA sequencing data. Considering the popularity of the tidyverse ecosystem, which offers a large set of data display, query, manipulation, integration and visualization utilities, a great opportunity exists to interface the Seurat object with the tidyverse. This interface gives the large data science community of tidyverse users the possibility to operate with familiar grammar. Results: To provide Seurat with a tidyverse-oriented interface without compromising efficiency, we developed tidyseurat, a lightweight adapter to the tidyverse. Tidyseurat displays cell information as a tibble abstraction, allowing intuitively interfacing Seurat with dplyr, tidyr, ggplot2 and plotly packages powering efficient data manipulation, integration and visualization. Iterative analyses on data subsets are enabled by interfacing with the popular nest-map framework.

Original languageEnglish
Pages (from-to)4100-4107
Number of pages8
JournalBioinformatics
Volume37
Issue number22
DOIs
Publication statusPublished - 15 Nov 2021
Externally publishedYes

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