Intracellular synthesis of silver nanoparticle by actinobacteria and its antimicrobial activity

S. V. Otari; R. M. Patil; S. J. Ghosh; N. D. Thorat; S. H. Pawar

doi:10.1016/j.saa.2014.10.003

Intracellular synthesis of silver nanoparticle by actinobacteria and its antimicrobial activity

S. V. Otari, R. M. Patil, S. J. Ghosh, N. D. Thorat, S. H. Pawar

Research output: Contribution to journal › Article › peer-review

Abstract

Intracellular synthesis of silver nanoparticles (AgNPs) using Rhodococcus spp. is demonstrated. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction, energy dispersive spectroscopy, Fourier trans-form infrared spectroscopy, and transmission electron microscopy. Transmission electron microscopy study of microorganisms' revealed synthesis of nanoparticle was occurring inside the cell, in the cytoplasm. AgNPs ranged from 5 to 50 nm. Formed nanoparticles were stable in the colloidal solution due to presence of proteins on the surface. AgNPs showed excellent bactericidal and bacteriostatic activity against pathogenic microorganisms.

Original language	English
Pages (from-to)	1175-1180
Number of pages	6
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	136
Issue number	PB
DOIs	https://doi.org/10.1016/j.saa.2014.10.003
Publication status	Published - 5 Feb 2015
Externally published	Yes

Keywords

Actinobacteria
AgNPs
DLS
Green synthesis
Intracellular synthesis

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10.1016/j.saa.2014.10.003

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title = "Intracellular synthesis of silver nanoparticle by actinobacteria and its antimicrobial activity",

abstract = "Intracellular synthesis of silver nanoparticles (AgNPs) using Rhodococcus spp. is demonstrated. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction, energy dispersive spectroscopy, Fourier trans-form infrared spectroscopy, and transmission electron microscopy. Transmission electron microscopy study of microorganisms' revealed synthesis of nanoparticle was occurring inside the cell, in the cytoplasm. AgNPs ranged from 5 to 50 nm. Formed nanoparticles were stable in the colloidal solution due to presence of proteins on the surface. AgNPs showed excellent bactericidal and bacteriostatic activity against pathogenic microorganisms.",

keywords = "Actinobacteria, AgNPs, DLS, Green synthesis, Intracellular synthesis",

author = "Otari, {S. V.} and Patil, {R. M.} and Ghosh, {S. J.} and Thorat, {N. D.} and Pawar, {S. H.}",

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T1 - Intracellular synthesis of silver nanoparticle by actinobacteria and its antimicrobial activity

AU - Otari, S. V.

AU - Patil, R. M.

AU - Ghosh, S. J.

AU - Thorat, N. D.

AU - Pawar, S. H.

PY - 2015/2/5

Y1 - 2015/2/5

N2 - Intracellular synthesis of silver nanoparticles (AgNPs) using Rhodococcus spp. is demonstrated. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction, energy dispersive spectroscopy, Fourier trans-form infrared spectroscopy, and transmission electron microscopy. Transmission electron microscopy study of microorganisms' revealed synthesis of nanoparticle was occurring inside the cell, in the cytoplasm. AgNPs ranged from 5 to 50 nm. Formed nanoparticles were stable in the colloidal solution due to presence of proteins on the surface. AgNPs showed excellent bactericidal and bacteriostatic activity against pathogenic microorganisms.

AB - Intracellular synthesis of silver nanoparticles (AgNPs) using Rhodococcus spp. is demonstrated. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction, energy dispersive spectroscopy, Fourier trans-form infrared spectroscopy, and transmission electron microscopy. Transmission electron microscopy study of microorganisms' revealed synthesis of nanoparticle was occurring inside the cell, in the cytoplasm. AgNPs ranged from 5 to 50 nm. Formed nanoparticles were stable in the colloidal solution due to presence of proteins on the surface. AgNPs showed excellent bactericidal and bacteriostatic activity against pathogenic microorganisms.

KW - Actinobacteria

KW - AgNPs

KW - DLS

KW - Green synthesis

KW - Intracellular synthesis

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DO - 10.1016/j.saa.2014.10.003

M3 - Article

C2 - 25456659

AN - SCOPUS:84911901266

SN - 1386-1425

VL - 136

SP - 1175

EP - 1180

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

IS - PB

ER -

Intracellular synthesis of silver nanoparticle by actinobacteria and its antimicrobial activity

Abstract

Keywords

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