Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning

Martijn A. Hoeben; Pim Van Hee; Rob G.J.M. Van Der Lans; Gerard Kwant; Luuk A.M. Van Der Wielen

doi:10.1002/bit.20653

Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning

Martijn A. Hoeben, Pim Van Hee, Rob G.J.M. Van Der Lans, Gerard Kwant, Luuk A.M. Van Der Wielen

Research output: Contribution to journal › Article › peer-review

Abstract

The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.

Original language	English
Pages (from-to)	607-617
Number of pages	11
Journal	Biotechnology and Bioengineering
Volume	93
Issue number	4
DOIs	https://doi.org/10.1002/bit.20653
Publication status	Published - 5 Mar 2006
Externally published	Yes

Keywords

Ampicillin
Crystals
Interfacial partitioning
Particle recovery
Phenylglycine
Solid-solid separation
Two-phase system

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10.1002/bit.20653

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title = "Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning",

abstract = "The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.",

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AU - Hoeben, Martijn A.

AU - Van Hee, Pim

AU - Van Der Lans, Rob G.J.M.

AU - Kwant, Gerard

AU - Van Der Wielen, Luuk A.M.

PY - 2006/3/5

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N2 - The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.

AB - The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.

KW - Ampicillin

KW - Crystals

KW - Interfacial partitioning

KW - Particle recovery

KW - Phenylglycine

KW - Solid-solid separation

KW - Two-phase system

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JO - Biotechnology and Bioengineering

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ER -

Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning

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Keywords

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