Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning

Martijn A. Hoeben; Pim Van Hee; Rob G.J.M. Van Der Lans; Gerard Kwant; Luuk A.M. Van Der Wielen

doi:10.1002/bit.20653

Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning

Martijn A. Hoeben
, Pim Van Hee
, Rob G.J.M. Van Der Lans
, Gerard Kwant
, Luuk A.M. Van Der Wielen

Research output: Contribution to journal › Article › peer-review

Abstract

The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.

Original language	English
Pages (from-to)	607-617
Number of pages	11
Journal	Biotechnology and Bioengineering
Volume	93
Issue number	4
DOIs	https://doi.org/10.1002/bit.20653
Publication status	Published - 5 Mar 2006
Externally published	Yes

Keywords

Ampicillin
Crystals
Interfacial partitioning
Particle recovery
Phenylglycine
Solid-solid separation
Two-phase system

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10.1002/bit.20653

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title = "Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning",

abstract = "The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.",

keywords = "Ampicillin, Crystals, Interfacial partitioning, Particle recovery, Phenylglycine, Solid-solid separation, Two-phase system",

author = "Hoeben, \{Martijn A.\} and \{Van Hee\}, Pim and \{Van Der Lans\}, \{Rob G.J.M.\} and Gerard Kwant and \{Van Der Wielen\}, \{Luuk A.M.\}",

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doi = "10.1002/bit.20653",

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T1 - Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning

AU - Hoeben, Martijn A.

AU - Van Hee, Pim

AU - Van Der Lans, Rob G.J.M.

AU - Kwant, Gerard

AU - Van Der Wielen, Luuk A.M.

PY - 2006/3/5

Y1 - 2006/3/5

N2 - The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.

AB - The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/ pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/ methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation.

KW - Ampicillin

KW - Crystals

KW - Interfacial partitioning

KW - Particle recovery

KW - Phenylglycine

KW - Solid-solid separation

KW - Two-phase system

UR - https://www.scopus.com/pages/publications/33644878895

U2 - 10.1002/bit.20653

DO - 10.1002/bit.20653

M3 - Article

C2 - 16395721

AN - SCOPUS:33644878895

SN - 0006-3592

VL - 93

SP - 607

EP - 617

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 4

ER -

Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning

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Keywords

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