TY - JOUR
T1 - Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
AU - Barreto, George Emílio Sampaio
AU - De Oliveira, Elineusa Silva
AU - De Villar, Flávio Augusto Guerreiro Aragão
AU - Dos Santos, Gleide Souza
AU - Lima, Rute Maria Ferreira
AU - Costa, Silvia Lima
AU - Costa, Maria De Fátima Dias
AU - Egito, Eryvaldo Sócrates Tabosa
AU - El-Bachá, Ramon Santos
PY - 2007/9
Y1 - 2007/9
N2 - The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 μM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH 2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 μM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.
AB - The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 μM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH 2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 μM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.
KW - 3-methylcatechol
KW - Astrocytes
KW - Brain
KW - Cytotoxicity
KW - Peroxidation
KW - Respiration
UR - http://www.scopus.com/inward/record.url?scp=37749026707&partnerID=8YFLogxK
U2 - 10.1590/S1516-89132007000500012
DO - 10.1590/S1516-89132007000500012
M3 - Article
AN - SCOPUS:37749026707
SN - 1516-8913
VL - 50
SP - 839
EP - 849
JO - Brazilian Archives of Biology and Technology
JF - Brazilian Archives of Biology and Technology
IS - 5
ER -