Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

George Emílio Sampaio Barreto; Elineusa Silva De Oliveira; Flávio Augusto Guerreiro Aragão De Villar; Gleide Souza Dos Santos; Rute Maria Ferreira Lima; Silvia Lima Costa; Maria De Fátima Dias Costa; Eryvaldo Sócrates Tabosa Egito; Ramon Santos El-Bachá

doi:10.1590/S1516-89132007000500012

Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

George Emílio Sampaio Barreto
, Elineusa Silva De Oliveira
, Flávio Augusto Guerreiro Aragão De Villar
, Gleide Souza Dos Santos
, Rute Maria Ferreira Lima
, Silvia Lima Costa
, Maria De Fátima Dias Costa
, Eryvaldo Sócrates Tabosa Egito
, Ramon Santos El-Bachá

Universidade Federal da Bahia
Universidade Federal do Rio Grande do Norte

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 μM.min^-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH ₂-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 μM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.

Original language	English
Pages (from-to)	839-849
Number of pages	11
Journal	Brazilian Archives of Biology and Technology
Volume	50
Issue number	5
DOIs	https://doi.org/10.1590/S1516-89132007000500012
Publication status	Published - Sep 2007
Externally published	Yes

Keywords

3-methylcatechol
Astrocytes
Brain
Cytotoxicity
Peroxidation
Respiration

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10.1590/S1516-89132007000500012

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Barreto, G. E. S., De Oliveira, E. S., De Villar, F. A. G. A., Dos Santos, G. S., Lima, R. M. F., Costa, S. L., Costa, M. D. F. D., Egito, E. S. T., & El-Bachá, R. S. (2007). Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol. Brazilian Archives of Biology and Technology, 50(5), 839-849. https://doi.org/10.1590/S1516-89132007000500012

@article{5103d882e5fd44baa9774287088b226b,

title = "Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol",

abstract = "The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 μM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH 2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50\% of cells after 72 h was 107 μM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.",

keywords = "3-methylcatechol, Astrocytes, Brain, Cytotoxicity, Peroxidation, Respiration",

author = "Barreto, \{George Em{\'i}lio Sampaio\} and \{De Oliveira\}, \{Elineusa Silva\} and \{De Villar\}, \{Fl{\'a}vio Augusto Guerreiro Arag{\~a}o\} and \{Dos Santos\}, \{Gleide Souza\} and Lima, \{Rute Maria Ferreira\} and Costa, \{Silvia Lima\} and Costa, \{Maria De F{\'a}tima Dias\} and Egito, \{Eryvaldo S{\'o}crates Tabosa\} and El-Bach{\'a}, \{Ramon Santos\}",

year = "2007",

month = sep,

doi = "10.1590/S1516-89132007000500012",

language = "English",

volume = "50",

pages = "839--849",

journal = "Brazilian Archives of Biology and Technology",

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TY - JOUR

T1 - Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

AU - Barreto, George Emílio Sampaio

AU - De Oliveira, Elineusa Silva

AU - De Villar, Flávio Augusto Guerreiro Aragão

AU - Dos Santos, Gleide Souza

AU - Lima, Rute Maria Ferreira

AU - Costa, Silvia Lima

AU - Costa, Maria De Fátima Dias

AU - Egito, Eryvaldo Sócrates Tabosa

AU - El-Bachá, Ramon Santos

PY - 2007/9

Y1 - 2007/9

N2 - The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 μM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH 2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 μM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.

AB - The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 μM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH 2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 μM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.

KW - 3-methylcatechol

KW - Astrocytes

KW - Brain

KW - Cytotoxicity

KW - Peroxidation

KW - Respiration

UR - https://www.scopus.com/pages/publications/37749026707

U2 - 10.1590/S1516-89132007000500012

DO - 10.1590/S1516-89132007000500012

M3 - Article

AN - SCOPUS:37749026707

SN - 1516-8913

VL - 50

SP - 839

EP - 849

JO - Brazilian Archives of Biology and Technology

JF - Brazilian Archives of Biology and Technology

IS - 5

ER -

Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

Abstract

Keywords

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