Abstract
A method of isolating in culture a specific subpopulation of cells in the "S" phase of the mitotic cycle, from embryonic forebrain, is described. Different enzyme treatment and different substratum pretreatment are compared quantitatively to ensure optimal yield. These cells, isolated from the telencephalic vesicles of embryonic day (E) 13 rat embryos, were maintained in culture and their morphological and immunocytochemical characteristics were investigated at one and eight days in vitro.
Original language | English |
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Pages (from-to) | 151-160 |
Number of pages | 10 |
Journal | Journal of Neuroscience Methods |
Volume | 38 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - Jul 1991 |
Externally published | Yes |
Keywords
- Central nervous system
- Development
- Immunocytochemistry
- Progenitor cells
- Rat
- Tissue culture