Isolation and characterisation of proliferating cells from the telencephalic vesicles of gestational-day 13 rats

H. Koppel, K. W. McDermott, P. L. Lantos

Research output: Contribution to journalArticlepeer-review

Abstract

A method of isolating in culture a specific subpopulation of cells in the "S" phase of the mitotic cycle, from embryonic forebrain, is described. Different enzyme treatment and different substratum pretreatment are compared quantitatively to ensure optimal yield. These cells, isolated from the telencephalic vesicles of embryonic day (E) 13 rat embryos, were maintained in culture and their morphological and immunocytochemical characteristics were investigated at one and eight days in vitro.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalJournal of Neuroscience Methods
Volume38
Issue number2-3
DOIs
Publication statusPublished - Jul 1991
Externally publishedYes

Keywords

  • Central nervous system
  • Development
  • Immunocytochemistry
  • Progenitor cells
  • Rat
  • Tissue culture

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