Isolation of a novel Thermus thermophilus metal efflux protein that improves Escherichia coli growth under stress conditions

Stefania Spada, J. Tony Pembroke, J. Gerard Wall

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanisms of metal ion transport in thermophilic organisms are poorly understood. Phage display-based screening of a Thermus thermophilus genomic library in Escherichia coli led to the identification of a novel metal cation efflux protein. The Thermus protein showed extensive sequence and putative structural conservation to Czr and Czc proteins in mesophilic bacterial and mammalian species. Expression of the gene in E. coli led to increased resistance to zinc and cadmium ions, but not to cobalt, in an effect that was apparently caused by increased efflux of metals from the cell. This increased resistance was inducible by zinc and cadmium and, to a lesser extent, by cobalt. Furthermore, E. coli cells containing the Thermus gene exhibited improved cell physiology and delayed cell lysis during recombinant protein production, leading to accumulation of higher levels of recombinant protein. The molecular basis and potential application of the findings are discussed.

Original languageEnglish
Pages (from-to)301-308
Number of pages8
JournalExtremophiles
Volume6
Issue number4
DOIs
Publication statusPublished - 2002

Keywords

  • CzrB
  • Heavy metal efflux
  • Stress reduction
  • Thermus thermophilus

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