Label free detection of specific protein binding using a microwave sensor

Marcela Salazar-Alvarez; Olga Korostynska; Alex Mason; Ahmed Al-Shamma'A; Jakki C. Cooney; Edmond Magner; Syed A.M. Tofail

doi:10.1039/c4an00909f

Label free detection of specific protein binding using a microwave sensor

Marcela Salazar-Alvarez, Olga Korostynska, Alex Mason, Ahmed Al-Shamma'A, Jakki C. Cooney, Edmond Magner, Syed A.M. Tofail

Research output: Contribution to journal › Article › peer-review

Abstract

The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.

Original language	English
Pages (from-to)	5335-5338
Number of pages	4
Journal	Analyst
Volume	139
Issue number	21
DOIs	https://doi.org/10.1039/c4an00909f
Publication status	Published - 2014

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title = "Label free detection of specific protein binding using a microwave sensor",

abstract = "The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.",

author = "Marcela Salazar-Alvarez and Olga Korostynska and Alex Mason and Ahmed Al-Shamma'A and Cooney, {Jakki C.} and Edmond Magner and Tofail, {Syed A.M.}",

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AU - Salazar-Alvarez, Marcela

AU - Korostynska, Olga

AU - Mason, Alex

AU - Al-Shamma'A, Ahmed

AU - Cooney, Jakki C.

AU - Magner, Edmond

AU - Tofail, Syed A.M.

N1 - Publisher Copyright: © the Partner Organisations 2014.

PY - 2014

Y1 - 2014

N2 - The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.

AB - The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.

UR - http://www.scopus.com/inward/record.url?scp=84908350533&partnerID=8YFLogxK

U2 - 10.1039/c4an00909f

DO - 10.1039/c4an00909f

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VL - 139

SP - 5335

EP - 5338

JO - Analyst

JF - Analyst

IS - 21

ER -

Label free detection of specific protein binding using a microwave sensor

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