Location and cloning of the UV sensitising function from the chromosomally associated incJ group plasmid R391

J Tony Pembroke; Evelyn Stevens; Jourica A. Brandsma; Pieter Van de Putte

doi:10.1016/0147-619X(86)90076-4

Location and cloning of the UV sensitising function from the chromosomally associated incJ group plasmid R391

J Tony Pembroke, Evelyn Stevens, Jourica A. Brandsma, Pieter Van de Putte

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Abstract

The IncJ plasmid R391, which specifies a uv-sensitizing function, has been shown to be associated with chromosomal DNA. Deletions originating from Tn10 insertion into the kanamycin-resistance determinant of plasmid R391 gave rise to uv-resistant derivatives. This apparent linkage between the kanamycin-resistance determinant and the uv-sensitizing gene(s) was used to clone the uv-sensitizing function from plasmid R391 into pUR222. A recombinant plasmid containing both functions (Kan^R and Uvs⁺) was obtained. The uv-sensitizing function was mapped to a 4-kb EcoRI fragment.

Original language	English (Ireland)
Pages (from-to)	30-36
Number of pages	7
Journal	Plasmid
Volume	16
Issue number	1
DOIs	https://doi.org/10.1016/0147-619X(86)90076-4
Publication status	Published - Jul 1986

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abstract = "The IncJ plasmid R391, which specifies a uv-sensitizing function, has been shown to be associated with chromosomal DNA. Deletions originating from Tn10 insertion into the kanamycin-resistance determinant of plasmid R391 gave rise to uv-resistant derivatives. This apparent linkage between the kanamycin-resistance determinant and the uv-sensitizing gene(s) was used to clone the uv-sensitizing function from plasmid R391 into pUR222. A recombinant plasmid containing both functions (KanR and Uvs+) was obtained. The uv-sensitizing function was mapped to a 4-kb EcoRI fragment.",

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AU - Van de Putte, Pieter

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AB - The IncJ plasmid R391, which specifies a uv-sensitizing function, has been shown to be associated with chromosomal DNA. Deletions originating from Tn10 insertion into the kanamycin-resistance determinant of plasmid R391 gave rise to uv-resistant derivatives. This apparent linkage between the kanamycin-resistance determinant and the uv-sensitizing gene(s) was used to clone the uv-sensitizing function from plasmid R391 into pUR222. A recombinant plasmid containing both functions (KanR and Uvs+) was obtained. The uv-sensitizing function was mapped to a 4-kb EcoRI fragment.

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Location and cloning of the UV sensitising function from the chromosomally associated incJ group plasmid R391

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