TY - JOUR
T1 - Mathematical modelling for the optimization of cellulase production using glycerol for cell growth and cellulose as the inducer substrate
AU - Gelain, Lucas
AU - van der Wielen, Luuk
AU - van Gulik, Walter M.
AU - Geraldo da Cruz Pradella, José
AU - Carvalho da Costa, Aline
N1 - Publisher Copyright:
© 2020 The Author(s)
PY - 2020/11
Y1 - 2020/11
N2 - Cellulase production can be divided into two steps: growth stage; followed by an induction stage. To develop a mathematical model for the optimization of this strategy, two sets of experiments were performed in batch mode for parameter estimation. One set of experiments was performed to evaluate the influence of glycerol regarding cell growth (initial concentrations of 5, 10, 15 and 20 g/L). The other set of experiments considered the induction stage using cellulose as the substrate (initial concentrations of 5, 10, 20, 30 and 40 g/L). Two feeding strategies were simulated to maximize cellulase production using glycerol to maintain a high cell concentration. The first simulation used a discrete feed and the second used a continuous feed of cellulose. The mathematical model proposed allows maintaining a high cell concentration and the addition of optimal small amounts of the inducer substrate to prevent inhibition of enzyme production.
AB - Cellulase production can be divided into two steps: growth stage; followed by an induction stage. To develop a mathematical model for the optimization of this strategy, two sets of experiments were performed in batch mode for parameter estimation. One set of experiments was performed to evaluate the influence of glycerol regarding cell growth (initial concentrations of 5, 10, 15 and 20 g/L). The other set of experiments considered the induction stage using cellulose as the substrate (initial concentrations of 5, 10, 20, 30 and 40 g/L). Two feeding strategies were simulated to maximize cellulase production using glycerol to maintain a high cell concentration. The first simulation used a discrete feed and the second used a continuous feed of cellulose. The mathematical model proposed allows maintaining a high cell concentration and the addition of optimal small amounts of the inducer substrate to prevent inhibition of enzyme production.
KW - Bioprocess
KW - Cellulose
KW - Glycerol
KW - Mathematical modelling
KW - Optimization of cellulase production
KW - Trichoderma harzianum
UR - http://www.scopus.com/inward/record.url?scp=85092717182&partnerID=8YFLogxK
U2 - 10.1016/j.cesx.2020.100085
DO - 10.1016/j.cesx.2020.100085
M3 - Article
AN - SCOPUS:85092717182
SN - 2590-1400
VL - 8
JO - Chemical Engineering Science: X
JF - Chemical Engineering Science: X
M1 - 100085
ER -