Measuring Albumen and Calcium in a dual test with the Hitachi 704

J Tony Pembroke; N. O. Leary; P. F. Duggan

Measuring Albumen and Calcium in a dual test with the Hitachi 704

J Tony Pembroke, N. O. Leary, P. F. Duggan

Retired Staff

University College Cork

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a method for simultaneously determining albumin, by using bromcresol purple, and calcium, by using Arsenazo III, in the same analytical cuvette on the Hitachi 704. Both assays agree well with accepted procedures. The standard curves for the albumin and calcium assays are linear from 0 to 60 g/L and 0 to 5.0 mmol/L, respectively. Calibration is stable for 7 days with use of open reagent in the instrument. Both assays are unaffected by hemoglobin ≤5 g/L and Intralipid ≤4 g/L; calcium is unaffected by bilirubin ≤600 μmol/L.

Original language	English (Ireland)
Pages (from-to)	1342-1345
Number of pages	4
Journal	Clinical Chemistry
Volume	38
Issue number	7
Publication status	Published - 1992

Keywords

Colorimetry
Multianalyte assay

Cite this

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abstract = "We describe a method for simultaneously determining albumin, by using bromcresol purple, and calcium, by using Arsenazo III, in the same analytical cuvette on the Hitachi 704. Both assays agree well with accepted procedures. The standard curves for the albumin and calcium assays are linear from 0 to 60 g/L and 0 to 5.0 mmol/L, respectively. Calibration is stable for 7 days with use of open reagent in the instrument. Both assays are unaffected by hemoglobin ≤5 g/L and Intralipid ≤4 g/L; calcium is unaffected by bilirubin ≤600 μmol/L.",

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N2 - We describe a method for simultaneously determining albumin, by using bromcresol purple, and calcium, by using Arsenazo III, in the same analytical cuvette on the Hitachi 704. Both assays agree well with accepted procedures. The standard curves for the albumin and calcium assays are linear from 0 to 60 g/L and 0 to 5.0 mmol/L, respectively. Calibration is stable for 7 days with use of open reagent in the instrument. Both assays are unaffected by hemoglobin ≤5 g/L and Intralipid ≤4 g/L; calcium is unaffected by bilirubin ≤600 μmol/L.

AB - We describe a method for simultaneously determining albumin, by using bromcresol purple, and calcium, by using Arsenazo III, in the same analytical cuvette on the Hitachi 704. Both assays agree well with accepted procedures. The standard curves for the albumin and calcium assays are linear from 0 to 60 g/L and 0 to 5.0 mmol/L, respectively. Calibration is stable for 7 days with use of open reagent in the instrument. Both assays are unaffected by hemoglobin ≤5 g/L and Intralipid ≤4 g/L; calcium is unaffected by bilirubin ≤600 μmol/L.

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KW - Multianalyte assay

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JO - Clinical Chemistry

JF - Clinical Chemistry

IS - 7

ER -

Measuring Albumen and Calcium in a dual test with the Hitachi 704

Abstract

Keywords

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