TY - JOUR
T1 - Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds
AU - Callanan, Anthony
AU - Davis, Niall F.
AU - Walsh, Michael T.
AU - McGloughlin, Timothy M.
N1 - Copyright © 2012 IPEM. Published by Elsevier Ltd. All rights reserved.
PY - 2012/11
Y1 - 2012/11
N2 - Multilayered biological scaffolds derived from mammalian extracellular matrix (ECM) have shown promising long-term clinical results when reconstructing damaged tissues and organs. Despite their established clinical applicability, experimental studies that describe the effects of alternate manufacturing protocols on an ECM's mechanical properties are lacking. In the present study the mechanical properties of multilayered 'unidirectional' porcine urinary bladder matrix (UBM) scaffolds were determined in favour of its longitudinal and circumferential axes. The scaffold's unidirectional mechanical properties were then compared with 'cross-directional' UBM scaffolds. The results showed significant variations when alternate manufacturing protocols for multilayered UBM were applied. Unidirectional longitudinal UBM remained the strongest biomaterial on a consistent basis. Its failure strength occurred at 4.79 ± 0.85. MPa compared to 3.36 ± 0.53. MPa for unidirectional circumferential and 2.91 ± 1.05. MPa for cross-directional UBM respectively (p< 0.0001). Distensibility was greatest in unidirectional circumferential UBM with failure extension occurring at 14.77 ± 1.66. mm. In comparison, failure extension occurred at 12.88 ± 0.94. mm and 13.04 ± 4.35. mm for unidirectional longitudinal and cross-directional UBM respectively (p= 0.0024). The present study demonstrates that predefined manufacturing protocols for UBM should be considered when reconstructing anatomical structures with specific mechanical requirements.
AB - Multilayered biological scaffolds derived from mammalian extracellular matrix (ECM) have shown promising long-term clinical results when reconstructing damaged tissues and organs. Despite their established clinical applicability, experimental studies that describe the effects of alternate manufacturing protocols on an ECM's mechanical properties are lacking. In the present study the mechanical properties of multilayered 'unidirectional' porcine urinary bladder matrix (UBM) scaffolds were determined in favour of its longitudinal and circumferential axes. The scaffold's unidirectional mechanical properties were then compared with 'cross-directional' UBM scaffolds. The results showed significant variations when alternate manufacturing protocols for multilayered UBM were applied. Unidirectional longitudinal UBM remained the strongest biomaterial on a consistent basis. Its failure strength occurred at 4.79 ± 0.85. MPa compared to 3.36 ± 0.53. MPa for unidirectional circumferential and 2.91 ± 1.05. MPa for cross-directional UBM respectively (p< 0.0001). Distensibility was greatest in unidirectional circumferential UBM with failure extension occurring at 14.77 ± 1.66. mm. In comparison, failure extension occurred at 12.88 ± 0.94. mm and 13.04 ± 4.35. mm for unidirectional longitudinal and cross-directional UBM respectively (p= 0.0024). The present study demonstrates that predefined manufacturing protocols for UBM should be considered when reconstructing anatomical structures with specific mechanical requirements.
KW - Extracellular matrix
KW - Mechanical properties
KW - Regenerative medicine
KW - Tissue engineering
KW - Urinary bladder matrix
UR - http://www.scopus.com/inward/record.url?scp=84867226808&partnerID=8YFLogxK
U2 - 10.1016/j.medengphy.2012.06.022
DO - 10.1016/j.medengphy.2012.06.022
M3 - Article
C2 - 22885225
AN - SCOPUS:84867226808
SN - 1350-4533
VL - 34
SP - 1368
EP - 1374
JO - Medical Engineering and Physics
JF - Medical Engineering and Physics
IS - 9
ER -