TY - JOUR
T1 - Mesoporous silica pellets as bifunctional bone drug delivery system for cefazolin
AU - Szewczyk, Adrian
AU - Skwira, Adrianna
AU - Konopacka, Agnieszka
AU - Sądej, Rafał
AU - Walker, Gavin
AU - Prokopowicz, Magdalena
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/10/15
Y1 - 2020/10/15
N2 - For decades, bone drug delivery systems dedicated for osteomyelitis treatment have been investigated as bifunctional materials that exhibit prolonged drug release and mineralization potential. Herein, composite-type pellets based on cefazolin-loaded amino-modified mesoporous silica SBA-15 and microwave-assisted hydroxyapatite were investigated as potential bone drug delivery system in vitro. Pellets were obtained by granulation, extrusion and spheronization methods in laboratory scale and studied in terms of physical properties, drug release, mineralization potential, antimicrobial activity and cytotoxicity towards human osteoblasts. The obtained pellets were characterized for hardness and friability which indicated the pellets durability during further investigations. Prolonged (5-day) release of cefazolin from pellets was observed. The pellets exhibited mineralization potential in simulated body fluid, i.e., a continuous layer of bone-like apatite was formed on the surface of pellets after 28 days of incubation. An antimicrobial assay of pellets revealed an antibacterial effect against Staphylococcus aureus strain during 6 days. No cytotoxic effects of pellets towards human osteoblasts were observed. The obtained results proved that proposed pellets appear to have potential applications as bone drug delivery systems.
AB - For decades, bone drug delivery systems dedicated for osteomyelitis treatment have been investigated as bifunctional materials that exhibit prolonged drug release and mineralization potential. Herein, composite-type pellets based on cefazolin-loaded amino-modified mesoporous silica SBA-15 and microwave-assisted hydroxyapatite were investigated as potential bone drug delivery system in vitro. Pellets were obtained by granulation, extrusion and spheronization methods in laboratory scale and studied in terms of physical properties, drug release, mineralization potential, antimicrobial activity and cytotoxicity towards human osteoblasts. The obtained pellets were characterized for hardness and friability which indicated the pellets durability during further investigations. Prolonged (5-day) release of cefazolin from pellets was observed. The pellets exhibited mineralization potential in simulated body fluid, i.e., a continuous layer of bone-like apatite was formed on the surface of pellets after 28 days of incubation. An antimicrobial assay of pellets revealed an antibacterial effect against Staphylococcus aureus strain during 6 days. No cytotoxic effects of pellets towards human osteoblasts were observed. The obtained results proved that proposed pellets appear to have potential applications as bone drug delivery systems.
KW - Cefazolin
KW - Hydroxyapatite
KW - Mesoporous silica
KW - Osteomyelitis
KW - Pellets
UR - http://www.scopus.com/inward/record.url?scp=85089088236&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2020.119718
DO - 10.1016/j.ijpharm.2020.119718
M3 - Article
C2 - 32750441
AN - SCOPUS:85089088236
SN - 0378-5173
VL - 588
SP - 119718
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
M1 - 119718
ER -