Micellar gradients in size-exclusion simulated moving bed chromatography

D. A. Horneman; M. Ottens; J. T.F. Keurentjes; L. A.M. van der Wielen

doi:10.1016/j.chroma.2006.01.134

Micellar gradients in size-exclusion simulated moving bed chromatography

D. A. Horneman, M. Ottens, J. T.F. Keurentjes, L. A.M. van der Wielen

Research output: Contribution to journal › Article › peer-review

Abstract

The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C₁₂E₂₃ as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.

Original language	English
Pages (from-to)	130-139
Number of pages	10
Journal	Journal of Chromatography A
Volume	1113
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2006.01.134
Publication status	Published - 28 Apr 2006
Externally published	Yes

Keywords

Bioseparation
Chromatography
Gradient elution
Protein purification
SEC
SMB
Surfactants

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10.1016/j.chroma.2006.01.134

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title = "Micellar gradients in size-exclusion simulated moving bed chromatography",

abstract = "The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.",

keywords = "Bioseparation, Chromatography, Gradient elution, Protein purification, SEC, SMB, Surfactants",

author = "Horneman, {D. A.} and M. Ottens and Keurentjes, {J. T.F.} and {van der Wielen}, {L. A.M.}",

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AU - Horneman, D. A.

AU - Ottens, M.

AU - Keurentjes, J. T.F.

AU - van der Wielen, L. A.M.

PY - 2006/4/28

Y1 - 2006/4/28

N2 - The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.

AB - The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.

KW - Bioseparation

KW - Chromatography

KW - Gradient elution

KW - Protein purification

KW - SEC

KW - SMB

KW - Surfactants

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SN - 0021-9673

VL - 1113

SP - 130

EP - 139

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Micellar gradients in size-exclusion simulated moving bed chromatography

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Keywords

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