Microbiota of Human Breast Tissue

Camilla Urbaniak; Joanne Cummins; Muriel Brackstone; Jean M. Macklaim; Gregory B. Gloor; Chwanrow K. Baban; Leslie Scott; Deidre M. O'Hanlon; Jeremy P. Burton; Kevin P. Francis; Mark Tangney; Gregor Reid

doi:10.1128/aem.00242-14

Microbiota of Human Breast Tissue

Camilla Urbaniak
, Joanne Cummins
, Muriel Brackstone
, Jean M. Macklaim
, Gregory B. Gloor
, Chwanrow K. Baban
, Leslie Scott
, Deidre M. O'Hanlon
, Jeremy P. Burton
, Kevin P. Francis
, Mark Tangney
, Gregor Reid

Research output: Contribution to journal › Article › peer-review

Abstract

ABSTRACT In recent years, a greater appreciation for the microbes inhabiting human body sites has emerged. In the female mammary gland, milk has been shown to contain bacterial species, ostensibly reaching the ducts from the skin. We decided to investigate whether there is a microbiome within the mammary tissue. Using 16S rRNA sequencing and culture, we analyzed breast tissue from 81 women with and without cancer in Canada and Ireland. A diverse population of bacteria was detected within tissue collected from sites all around the breast in women aged 18 to 90, not all of whom had a history of lactation. The principal phylum was Proteobacteria . The most abundant taxa in the Canadian samples were Bacillus (11.4%), Acinetobacter (10.0%), Enterobacteriaceae (8.3%), Pseudomonas (6.5%), Staphylococcus (6.5%), Propionibacterium (5.8%), Comamonadaceae (5.7%), Gammaproteobacteria (5.0%), and Prevotella (5.0%). In the Irish samples the most abundant taxa were Enterobacteriaceae (30.8%), Staphylococcus (12.7%), Listeria welshimeri (12.1%), Propionibacterium (10.1%), and Pseudomonas (5.3%). None of the subjects had signs or symptoms of infection, but the presence of viable bacteria was confirmed in some samples by culture. The extent to which these organisms play a role in health or disease remains to be determined.

Original language	English
Journal	Applied and Environmental Microbiology
DOIs	https://doi.org/10.1128/aem.00242-14
Publication status	Published - 15 May 2014

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title = "Microbiota of Human Breast Tissue",

abstract = "ABSTRACT In recent years, a greater appreciation for the microbes inhabiting human body sites has emerged. In the female mammary gland, milk has been shown to contain bacterial species, ostensibly reaching the ducts from the skin. We decided to investigate whether there is a microbiome within the mammary tissue. Using 16S rRNA sequencing and culture, we analyzed breast tissue from 81 women with and without cancer in Canada and Ireland. A diverse population of bacteria was detected within tissue collected from sites all around the breast in women aged 18 to 90, not all of whom had a history of lactation. The principal phylum was Proteobacteria . The most abundant taxa in the Canadian samples were Bacillus (11.4\%), Acinetobacter (10.0\%), Enterobacteriaceae (8.3\%), Pseudomonas (6.5\%), Staphylococcus (6.5\%), Propionibacterium (5.8\%), Comamonadaceae (5.7\%), Gammaproteobacteria (5.0\%), and Prevotella (5.0\%). In the Irish samples the most abundant taxa were Enterobacteriaceae (30.8\%), Staphylococcus (12.7\%), Listeria welshimeri (12.1\%), Propionibacterium (10.1\%), and Pseudomonas (5.3\%). None of the subjects had signs or symptoms of infection, but the presence of viable bacteria was confirmed in some samples by culture. The extent to which these organisms play a role in health or disease remains to be determined. ",

author = "Camilla Urbaniak and Joanne Cummins and Muriel Brackstone and Macklaim, \{Jean M.\} and Gloor, \{Gregory B.\} and Baban, \{Chwanrow K.\} and Leslie Scott and O'Hanlon, \{Deidre M.\} and Burton, \{Jeremy P.\} and Francis, \{Kevin P.\} and Mark Tangney and Gregor Reid",

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doi = "10.1128/aem.00242-14",

language = "English",

journal = "Applied and Environmental Microbiology",

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T1 - Microbiota of Human Breast Tissue

AU - Urbaniak, Camilla

AU - Cummins, Joanne

AU - Brackstone, Muriel

AU - Macklaim, Jean M.

AU - Gloor, Gregory B.

AU - Baban, Chwanrow K.

AU - Scott, Leslie

AU - O'Hanlon, Deidre M.

AU - Burton, Jeremy P.

AU - Francis, Kevin P.

AU - Tangney, Mark

AU - Reid, Gregor

PY - 2014/5/15

Y1 - 2014/5/15

N2 - ABSTRACT In recent years, a greater appreciation for the microbes inhabiting human body sites has emerged. In the female mammary gland, milk has been shown to contain bacterial species, ostensibly reaching the ducts from the skin. We decided to investigate whether there is a microbiome within the mammary tissue. Using 16S rRNA sequencing and culture, we analyzed breast tissue from 81 women with and without cancer in Canada and Ireland. A diverse population of bacteria was detected within tissue collected from sites all around the breast in women aged 18 to 90, not all of whom had a history of lactation. The principal phylum was Proteobacteria . The most abundant taxa in the Canadian samples were Bacillus (11.4%), Acinetobacter (10.0%), Enterobacteriaceae (8.3%), Pseudomonas (6.5%), Staphylococcus (6.5%), Propionibacterium (5.8%), Comamonadaceae (5.7%), Gammaproteobacteria (5.0%), and Prevotella (5.0%). In the Irish samples the most abundant taxa were Enterobacteriaceae (30.8%), Staphylococcus (12.7%), Listeria welshimeri (12.1%), Propionibacterium (10.1%), and Pseudomonas (5.3%). None of the subjects had signs or symptoms of infection, but the presence of viable bacteria was confirmed in some samples by culture. The extent to which these organisms play a role in health or disease remains to be determined.

AB - ABSTRACT In recent years, a greater appreciation for the microbes inhabiting human body sites has emerged. In the female mammary gland, milk has been shown to contain bacterial species, ostensibly reaching the ducts from the skin. We decided to investigate whether there is a microbiome within the mammary tissue. Using 16S rRNA sequencing and culture, we analyzed breast tissue from 81 women with and without cancer in Canada and Ireland. A diverse population of bacteria was detected within tissue collected from sites all around the breast in women aged 18 to 90, not all of whom had a history of lactation. The principal phylum was Proteobacteria . The most abundant taxa in the Canadian samples were Bacillus (11.4%), Acinetobacter (10.0%), Enterobacteriaceae (8.3%), Pseudomonas (6.5%), Staphylococcus (6.5%), Propionibacterium (5.8%), Comamonadaceae (5.7%), Gammaproteobacteria (5.0%), and Prevotella (5.0%). In the Irish samples the most abundant taxa were Enterobacteriaceae (30.8%), Staphylococcus (12.7%), Listeria welshimeri (12.1%), Propionibacterium (10.1%), and Pseudomonas (5.3%). None of the subjects had signs or symptoms of infection, but the presence of viable bacteria was confirmed in some samples by culture. The extent to which these organisms play a role in health or disease remains to be determined.

UR - http://dx.doi.org/10.1128/aem.00242-14

U2 - 10.1128/aem.00242-14

DO - 10.1128/aem.00242-14

M3 - Article

SN - 0099-2240

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

ER -

Microbiota of Human Breast Tissue

Abstract

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