@inproceedings{fb243c99831e4c9c92c83dde5aacc207,
title = "Microchannel fluorescent melting curve analysis",
abstract = "In recent years, Fluorescent Melting Curve Analysis (FMCA) has become an almost ubiquitous feature of commercial quantitative PCR (qPCR) thermal cyclers. Here a microfluidic device is presented capable of performing droplet based Fluorescent Melting Curve Analysis within a microchannel. The device consists of modular thermally conductive blocks which can sandwich a microfluidic substrate. Opposing ends of the blocks are held at differing temperatures and a linear thermal gradient is generated along the microfluidic channel. Fluorescent measurements taken from a sample as it passes along the micro-fluidic channel permits fluorescent melting curves be generated. In this study we demonstrate the capability of measuring fluorescent melt curves from DNA passing through the device in a serial flow of sample plugs buffered by mineral oil.",
author = "Kinahan, {David J.} and Dalton, {Tara M.} and Davies, {Mark R.}",
year = "2008",
doi = "10.1115/ICNMM2008-62014",
language = "English",
isbn = "0791848345",
series = "Proceedings of the 6th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM2008",
number = "PART B",
pages = "1579--1585",
booktitle = "Proceedings of the 6th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM2008",
edition = "PART B",
note = "6th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM2008 ; Conference date: 23-06-2008 Through 25-06-2008",
}