Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition

Mohammad Ali Khosravi Zanjani, Babak Ghiassi Tarzi, Anousheh Sharifan, Nima Mohammadi

Research output: Contribution to journalArticlepeer-review

Abstract

Microencapsulation as one of the most modern methods has considerable effects on probiotic survival. In this study Lactobacillus casei (ATCC 39392) and Bifidobacterium bifidum (ATCC 29521) were encapsulated using calcium alginate-gelatinized starch, chitosan coating and inulin via emulsion technique, and were incubated in simulated gastric juice (along with pepsin, pH=1.5) and simulated intestinal juice (along with pancreatin and bile salts, pH = 8) for 2 hours at 37 °C. The morphology and size of microcapsules were measured by scanning electron and optical microscopy. The results indicated that the survival of microencapsulated probiotic increased significantly in simulated gastro-intestinal condition (P < 0.05). Chitosan coating played a significant role in the protection of probiotic bacteria in simulated gastro-intestinal condition and the diameter of the microcapsules increased with the addition of chitosan coating. In general, this study indicated that microencapsulation with alginate-gelatinized starch coated with chitosan could successfully and significantly protect probiotic bacteria against adverse condition of simulated human gastro-intestinal condition.

Original languageEnglish
Pages (from-to)843-852
Number of pages10
JournalIranian Journal of Pharmaceutical Research
Volume13
Issue number3
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Calcium alginate-gelatinized starch
  • Chitosan
  • Inulin
  • Microencapsulation
  • Simulated gastro-intestinal condition

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