Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol

Clíona M. McCarthy; Kevin L. McKevitt; Sinéad A. Connolly; Isabel Andersson; Fiona C. Leahy; Siobhan Egan; Michael A. Moloney; Eamon G. Kavanagh; Colin Peirce; Eoghan M. Cunnane; Kieran D. McGourty; Michael T. Walsh; John J.E. Mulvihill

doi:10.1371/journal.pone.0297618

Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol

Clíona M. McCarthy, Kevin L. McKevitt, Sinéad A. Connolly, Isabel Andersson, Fiona C. Leahy, Siobhan Egan, Michael A. Moloney, Eamon G. Kavanagh, Colin Peirce, Eoghan M. Cunnane, Kieran D. McGourty, Michael T. Walsh, John J.E. Mulvihill

Research output: Contribution to journal › Article › peer-review

Abstract

Microindentation of fresh biological tissues is necessary for the creation of 3D biomimetic models that accurately represent the native extracellular matrix microenvironment. However, tissue must first be precisely sectioned into slices. Challenges exist in the preparation of fresh tissue slices, as they can tear easily and must be processed rapidly in order to mitigate tissue degradation. In this study, we propose an optimised mounting condition for microindentation and demonstrate that embedding tissue in a mixture of 2.5% agarose and 1.5% gelatin is the most favourable method of tissue slice mounting for microindentation. This protocol allows for rapid processing of fresh biological tissue and is applicable to a variety of tissue types.

Original language	English
Article number	e0297618
Pages (from-to)	e0297618
Journal	PLoS ONE
Volume	19
Issue number	2 February
DOIs	https://doi.org/10.1371/journal.pone.0297618
Publication status	Published - Feb 2024

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McCarthy, C. M., McKevitt, K. L., Connolly, S. A., Andersson, I., Leahy, F. C., Egan, S., Moloney, M. A., Kavanagh, E. G., Peirce, C., Cunnane, E. M., McGourty, K. D., Walsh, M. T., & Mulvihill, J. J. E. (2024). Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol. PLoS ONE, 19(2 February), e0297618. Article e0297618. https://doi.org/10.1371/journal.pone.0297618

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abstract = "Microindentation of fresh biological tissues is necessary for the creation of 3D biomimetic models that accurately represent the native extracellular matrix microenvironment. However, tissue must first be precisely sectioned into slices. Challenges exist in the preparation of fresh tissue slices, as they can tear easily and must be processed rapidly in order to mitigate tissue degradation. In this study, we propose an optimised mounting condition for microindentation and demonstrate that embedding tissue in a mixture of 2.5% agarose and 1.5% gelatin is the most favourable method of tissue slice mounting for microindentation. This protocol allows for rapid processing of fresh biological tissue and is applicable to a variety of tissue types.",

author = "McCarthy, {Cl{\'i}ona M.} and McKevitt, {Kevin L.} and Connolly, {Sin{\'e}ad A.} and Isabel Andersson and Leahy, {Fiona C.} and Siobhan Egan and Moloney, {Michael A.} and Kavanagh, {Eamon G.} and Colin Peirce and Cunnane, {Eoghan M.} and McGourty, {Kieran D.} and Walsh, {Michael T.} and Mulvihill, {John J.E.}",

note = "Publisher Copyright: {\textcopyright} 2024 McCarthy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

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McCarthy, CM, McKevitt, KL, Connolly, SA, Andersson, I, Leahy, FC, Egan, S, Moloney, MA, Kavanagh, EG, Peirce, C, Cunnane, EM , McGourty, KD , Walsh, MT & Mulvihill, JJE 2024, 'Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol', PLoS ONE, vol. 19, no. 2 February, e0297618, pp. e0297618. https://doi.org/10.1371/journal.pone.0297618

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AU - Andersson, Isabel

AU - Leahy, Fiona C.

AU - Egan, Siobhan

AU - Moloney, Michael A.

AU - Kavanagh, Eamon G.

AU - Peirce, Colin

AU - Cunnane, Eoghan M.

AU - McGourty, Kieran D.

AU - Walsh, Michael T.

AU - Mulvihill, John J.E.

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Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol

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