TY - JOUR
T1 - Milk protein-derived peptides induce 5-HT2C-mediated satiety invivo
AU - Schellekens, Harriët
AU - Nongonierma, Alice B.
AU - Clarke, Gerard
AU - van Oeffelen, Wesley E.P.A.
AU - FitzGerald, Richard J.
AU - Dinan, Timothy G.
AU - Cryan, John F.
PY - 2014/9
Y1 - 2014/9
N2 - This study investigates the ability of milk protein-derived peptides to specifically activate the serotonin 2C (5-HT2C) receptor, a key receptor in central regulation of food intake. A dose dependent 5-HT2C receptor activation by the 1kDa ultrafiltration permeates of a sodium caseinate (NaCNH-1kDa permeate) and a whey protein hydrolysate (WPH-1kDa permeate) was demonstrated using an intracellular calcium mobilisation assay in human embryonic kidney (Hek) cells expressing the 5-HT2C receptor. Both samples activated the 5-HT2C but not the 5-HT2A and 5-HT2B receptors. NaCNH-1kDa permeate significantly (p<0.01) reduced cumulative food intake when administered to male mice (C57Bl/6) by intraperitoneal injection at 500mgkg-1 body weight. In contrast, no effect of WPH-1kDa permeate could be seen on food intake invivo. These results demonstrate the promising appetite-suppressing potential of NaCN-derived peptides, targeting the 5-HT2C receptor.
AB - This study investigates the ability of milk protein-derived peptides to specifically activate the serotonin 2C (5-HT2C) receptor, a key receptor in central regulation of food intake. A dose dependent 5-HT2C receptor activation by the 1kDa ultrafiltration permeates of a sodium caseinate (NaCNH-1kDa permeate) and a whey protein hydrolysate (WPH-1kDa permeate) was demonstrated using an intracellular calcium mobilisation assay in human embryonic kidney (Hek) cells expressing the 5-HT2C receptor. Both samples activated the 5-HT2C but not the 5-HT2A and 5-HT2B receptors. NaCNH-1kDa permeate significantly (p<0.01) reduced cumulative food intake when administered to male mice (C57Bl/6) by intraperitoneal injection at 500mgkg-1 body weight. In contrast, no effect of WPH-1kDa permeate could be seen on food intake invivo. These results demonstrate the promising appetite-suppressing potential of NaCN-derived peptides, targeting the 5-HT2C receptor.
UR - http://www.scopus.com/inward/record.url?scp=84901197032&partnerID=8YFLogxK
U2 - 10.1016/j.idairyj.2014.04.004
DO - 10.1016/j.idairyj.2014.04.004
M3 - Article
AN - SCOPUS:84901197032
SN - 0958-6946
VL - 38
SP - 55
EP - 64
JO - International Dairy Journal
JF - International Dairy Journal
IS - 1
ER -