Milk protein-derived peptides induce 5-HT2C-mediated satiety invivo

Harriët Schellekens; Alice B. Nongonierma; Gerard Clarke; Wesley E.P.A. van Oeffelen; Richard J. FitzGerald; Timothy G. Dinan; John F. Cryan

doi:10.1016/j.idairyj.2014.04.004

Milk protein-derived peptides induce 5-HT_2C-mediated satiety invivo

Harriët Schellekens
, Alice B. Nongonierma
, Gerard Clarke
, Wesley E.P.A. van Oeffelen
, Richard J. FitzGerald
, Timothy G. Dinan
, John F. Cryan

Research output: Contribution to journal › Article › peer-review

Abstract

This study investigates the ability of milk protein-derived peptides to specifically activate the serotonin 2C (5-HT_2C) receptor, a key receptor in central regulation of food intake. A dose dependent 5-HT_2C receptor activation by the 1kDa ultrafiltration permeates of a sodium caseinate (NaCNH-1kDa permeate) and a whey protein hydrolysate (WPH-1kDa permeate) was demonstrated using an intracellular calcium mobilisation assay in human embryonic kidney (Hek) cells expressing the 5-HT_2C receptor. Both samples activated the 5-HT_2C but not the 5-HT_2A and 5-HT_2B receptors. NaCNH-1kDa permeate significantly (p<0.01) reduced cumulative food intake when administered to male mice (C57Bl/6) by intraperitoneal injection at 500mgkg^-1 body weight. In contrast, no effect of WPH-1kDa permeate could be seen on food intake invivo. These results demonstrate the promising appetite-suppressing potential of NaCN-derived peptides, targeting the 5-HT_2C receptor.

Original language	English
Pages (from-to)	55-64
Number of pages	10
Journal	International Dairy Journal
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.idairyj.2014.04.004
Publication status	Published - Sep 2014

Access to Document

10.1016/j.idairyj.2014.04.004

Cite this

@article{cc49ee62f8934a0f944c58c584976004,

title = "Milk protein-derived peptides induce 5-HT2C-mediated satiety invivo",

abstract = "This study investigates the ability of milk protein-derived peptides to specifically activate the serotonin 2C (5-HT2C) receptor, a key receptor in central regulation of food intake. A dose dependent 5-HT2C receptor activation by the 1kDa ultrafiltration permeates of a sodium caseinate (NaCNH-1kDa permeate) and a whey protein hydrolysate (WPH-1kDa permeate) was demonstrated using an intracellular calcium mobilisation assay in human embryonic kidney (Hek) cells expressing the 5-HT2C receptor. Both samples activated the 5-HT2C but not the 5-HT2A and 5-HT2B receptors. NaCNH-1kDa permeate significantly (p<0.01) reduced cumulative food intake when administered to male mice (C57Bl/6) by intraperitoneal injection at 500mgkg-1 body weight. In contrast, no effect of WPH-1kDa permeate could be seen on food intake invivo. These results demonstrate the promising appetite-suppressing potential of NaCN-derived peptides, targeting the 5-HT2C receptor.",

author = "Harri{\"e}t Schellekens and Nongonierma, \{Alice B.\} and Gerard Clarke and \{van Oeffelen\}, \{Wesley E.P.A.\} and FitzGerald, \{Richard J.\} and Dinan, \{Timothy G.\} and Cryan, \{John F.\}",

year = "2014",

month = sep,

doi = "10.1016/j.idairyj.2014.04.004",

language = "English",

volume = "38",

pages = "55--64",

journal = "International Dairy Journal",

issn = "0958-6946",

number = "1",

}

TY - JOUR

T1 - Milk protein-derived peptides induce 5-HT2C-mediated satiety invivo

AU - Schellekens, Harriët

AU - Nongonierma, Alice B.

AU - Clarke, Gerard

AU - van Oeffelen, Wesley E.P.A.

AU - FitzGerald, Richard J.

AU - Dinan, Timothy G.

AU - Cryan, John F.

PY - 2014/9

Y1 - 2014/9

N2 - This study investigates the ability of milk protein-derived peptides to specifically activate the serotonin 2C (5-HT2C) receptor, a key receptor in central regulation of food intake. A dose dependent 5-HT2C receptor activation by the 1kDa ultrafiltration permeates of a sodium caseinate (NaCNH-1kDa permeate) and a whey protein hydrolysate (WPH-1kDa permeate) was demonstrated using an intracellular calcium mobilisation assay in human embryonic kidney (Hek) cells expressing the 5-HT2C receptor. Both samples activated the 5-HT2C but not the 5-HT2A and 5-HT2B receptors. NaCNH-1kDa permeate significantly (p<0.01) reduced cumulative food intake when administered to male mice (C57Bl/6) by intraperitoneal injection at 500mgkg-1 body weight. In contrast, no effect of WPH-1kDa permeate could be seen on food intake invivo. These results demonstrate the promising appetite-suppressing potential of NaCN-derived peptides, targeting the 5-HT2C receptor.

AB - This study investigates the ability of milk protein-derived peptides to specifically activate the serotonin 2C (5-HT2C) receptor, a key receptor in central regulation of food intake. A dose dependent 5-HT2C receptor activation by the 1kDa ultrafiltration permeates of a sodium caseinate (NaCNH-1kDa permeate) and a whey protein hydrolysate (WPH-1kDa permeate) was demonstrated using an intracellular calcium mobilisation assay in human embryonic kidney (Hek) cells expressing the 5-HT2C receptor. Both samples activated the 5-HT2C but not the 5-HT2A and 5-HT2B receptors. NaCNH-1kDa permeate significantly (p<0.01) reduced cumulative food intake when administered to male mice (C57Bl/6) by intraperitoneal injection at 500mgkg-1 body weight. In contrast, no effect of WPH-1kDa permeate could be seen on food intake invivo. These results demonstrate the promising appetite-suppressing potential of NaCN-derived peptides, targeting the 5-HT2C receptor.

UR - https://www.scopus.com/pages/publications/84901197032

U2 - 10.1016/j.idairyj.2014.04.004

DO - 10.1016/j.idairyj.2014.04.004

M3 - Article

AN - SCOPUS:84901197032

SN - 0958-6946

VL - 38

SP - 55

EP - 64

JO - International Dairy Journal

JF - International Dairy Journal

IS - 1

ER -

Milk protein-derived peptides induce 5-HT_2C-mediated satiety invivo

Abstract

Access to Document

Other files and links

Fingerprint

Cite this