@inproceedings{2f66099314844e3790a39b25fbe5e7d1,
title = "Monitoring of yeast cell concentration using a micromachined impedance sensor",
abstract = "This paper describes the design, modelling perimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces Cerevisiae cell culture show that the permittivity of the cell suspension depends linearly on the biomass concentration within the range of 0 to 9 g/1/. In order to compensate the measurements for changes in the dielectric properties of the background electrolyte, the use of a three-electrode configuration in combination with a semi-permeable pHEMA membrane was explored. Measurements showed that the impedance of the hydrated pHEMA varies with only the background electrolyte conductivity, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite element modelling.",
keywords = "Cell concentration, Dielectric spectroscopy, Microporous membrane, Permittivity, PHEMA",
author = "Krommenhoek, {E. E.} and Gardeniers, {J. G.E.} and Bomer, {J. G.} and {Van Den Berg}, A. and X. Li and M. Ottens and {Van Der Wielen}, {L. A.M.} and {Van Dedem}, {G. W.K.} and {Van Leeuwen}, M. and {Van Gulik}, {W. M.} and Heijnen, {J. J.}",
year = "2005",
language = "English",
isbn = "0780389948",
series = "Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05",
pages = "283--286",
booktitle = "TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers",
note = "13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 ; Conference date: 05-06-2005 Through 09-06-2005",
}