Monitoring of yeast cell concentration using a micromachined impedance sensor

E. E. Krommenhoek; J. G.E. Gardeniers; J. G. Bomer; A. Van den Berg; X. Li; M. Ottens; L. A.M. van der Wielen; G. W.K. van Dedem; M. Van Leeuwen; W. M. van Gulik; J. J. Heijnen

doi:10.1016/j.snb.2005.09.028

Monitoring of yeast cell concentration using a micromachined impedance sensor

E. E. Krommenhoek, J. G.E. Gardeniers, J. G. Bomer, A. Van den Berg, X. Li, M. Ottens, L. A.M. van der Wielen, G. W.K. van Dedem, M. Van Leeuwen, W. M. van Gulik, J. J. Heijnen

Research output: Contribution to journal › Article › peer-review

Abstract

This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the β-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 μm for the electrode configuration under study.

Original language	English
Pages (from-to)	384-389
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	115
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2005.09.028
Publication status	Published - 23 May 2006
Externally published	Yes

Keywords

Cell concentration
Dielectric spectroscopy
Microporous membrane
Permittivity
pHEMA

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10.1016/j.snb.2005.09.028

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Krommenhoek, E. E., Gardeniers, J. G. E., Bomer, J. G., Van den Berg, A., Li, X., Ottens, M., van der Wielen, L. A. M., van Dedem, G. W. K., Van Leeuwen, M., van Gulik, W. M., & Heijnen, J. J. (2006). Monitoring of yeast cell concentration using a micromachined impedance sensor. Sensors and Actuators, B: Chemical, 115(1), 384-389. https://doi.org/10.1016/j.snb.2005.09.028

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abstract = "This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the β-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 μm for the electrode configuration under study.",

keywords = "Cell concentration, Dielectric spectroscopy, Microporous membrane, Permittivity, pHEMA",

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AU - Krommenhoek, E. E.

AU - Gardeniers, J. G.E.

AU - Bomer, J. G.

AU - Van den Berg, A.

AU - Li, X.

AU - Ottens, M.

AU - van der Wielen, L. A.M.

AU - van Dedem, G. W.K.

AU - Van Leeuwen, M.

AU - van Gulik, W. M.

AU - Heijnen, J. J.

PY - 2006/5/23

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N2 - This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the β-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 μm for the electrode configuration under study.

AB - This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the β-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 μm for the electrode configuration under study.

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Monitoring of yeast cell concentration using a micromachined impedance sensor

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Keywords

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