TY - JOUR
T1 - Multifunctional nano-magnetic particles assisted viral RNA-extraction protocol for potential detection of COVID-19
AU - B. Somvanshi, Sandeep
AU - B. Kharat, Prashant
AU - S. Saraf, Tukaram
AU - B. Somwanshi, Saurabh
AU - B. Shejul, Sumit
AU - M. Jadhav, Kamalakar
N1 - Publisher Copyright:
© 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2020
Y1 - 2020
N2 - The enduring outbreak of the corona virus disease (COVID-19) originated from China illustrates global concerns owing to their infective nature and time-consuming incubation. Even though diagnostic tools based on RT-PCR are being extensively employed at present, well-timed and precise diagnostics are still restricted because of the long-period and exhaustive man-power requirement. To tackle this problem, we herewith report the fabrication of the surface functionalised magnetic nanoparticles (MNP’s) and viral RNA-extraction protocol for potential detection of COVID-19. The zinc ferrite nanoparticles were prepared by combustion synthesis and its surface was functionalised by silica and carboxyl-modified polyvinyl alcohol. The MNP’s were characterized to confirm the nano-scale appearance and functionalization. The proposed model may provide the ability to extract the viral RNA from several specimens through automation process. In light of ease and proficiency, this protocol may significantly lessen the operation period and necessities for the present molecular-level diagnostic of COVID-19.
AB - The enduring outbreak of the corona virus disease (COVID-19) originated from China illustrates global concerns owing to their infective nature and time-consuming incubation. Even though diagnostic tools based on RT-PCR are being extensively employed at present, well-timed and precise diagnostics are still restricted because of the long-period and exhaustive man-power requirement. To tackle this problem, we herewith report the fabrication of the surface functionalised magnetic nanoparticles (MNP’s) and viral RNA-extraction protocol for potential detection of COVID-19. The zinc ferrite nanoparticles were prepared by combustion synthesis and its surface was functionalised by silica and carboxyl-modified polyvinyl alcohol. The MNP’s were characterized to confirm the nano-scale appearance and functionalization. The proposed model may provide the ability to extract the viral RNA from several specimens through automation process. In light of ease and proficiency, this protocol may significantly lessen the operation period and necessities for the present molecular-level diagnostic of COVID-19.
KW - COVID-19
KW - Multifunctional MNP’s
KW - RNA-extraction
KW - surface functionalisation
UR - https://www.scopus.com/pages/publications/85085338991
U2 - 10.1080/14328917.2020.1769350
DO - 10.1080/14328917.2020.1769350
M3 - Article
AN - SCOPUS:85085338991
SN - 1432-8917
SP - 1
EP - 6
JO - Materials Research Innovations
JF - Materials Research Innovations
ER -
