TY - JOUR
T1 - Multilayered polyelectrolyte microcapsules
T2 - Interaction with the enzyme cytochrome c oxidase
AU - Pastorino, Laura
AU - Dellacasa, Elena
AU - Noor, Mohamed R.
AU - Soulimane, Tewfik
AU - Bianchini, Paolo
AU - D'Autilia, Francesca
AU - Antipov, Alexei
AU - Diaspro, Alberto
AU - Tofail, Syed A.M.
AU - Ruggiero, Carmelina
N1 - Publisher Copyright:
© 2014 Pastorino et al.
PY - 2014/11/5
Y1 - 2014/11/5
N2 - Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.
AB - Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.
UR - http://www.scopus.com/inward/record.url?scp=84910642768&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0112192
DO - 10.1371/journal.pone.0112192
M3 - Article
C2 - 25372607
AN - SCOPUS:84910642768
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 11
M1 - e112192
ER -