Multilayered polyelectrolyte microcapsules: Interaction with the enzyme cytochrome c oxidase

Laura Pastorino; Elena Dellacasa; Mohamed R. Noor; Tewfik Soulimane; Paolo Bianchini; Francesca D'Autilia; Alexei Antipov; Alberto Diaspro; Syed A.M. Tofail; Carmelina Ruggiero

doi:10.1371/journal.pone.0112192

Multilayered polyelectrolyte microcapsules: Interaction with the enzyme cytochrome c oxidase

Laura Pastorino, Elena Dellacasa, Mohamed R. Noor, Tewfik Soulimane, Paolo Bianchini, Francesca D'Autilia, Alexei Antipov, Alberto Diaspro, Syed A.M. Tofail, Carmelina Ruggiero

Research output: Contribution to journal › Article › peer-review

Abstract

Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

Original language	English
Article number	e112192
Pages (from-to)	e112192
Journal	PLoS ONE
Volume	9
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0112192
Publication status	Published - 5 Nov 2014

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abstract = "Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.",

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AU - Dellacasa, Elena

AU - Noor, Mohamed R.

AU - Soulimane, Tewfik

AU - Bianchini, Paolo

AU - D'Autilia, Francesca

AU - Antipov, Alexei

AU - Diaspro, Alberto

AU - Tofail, Syed A.M.

AU - Ruggiero, Carmelina

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AB - Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

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Multilayered polyelectrolyte microcapsules: Interaction with the enzyme cytochrome c oxidase

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