Multilayered polyelectrolyte microcapsules: Interaction with the enzyme cytochrome c oxidase

Laura Pastorino; Elena Dellacasa; Mohamed R. Noor; Tewfik Soulimane; Paolo Bianchini; Francesca D'Autilia; Alexei Antipov; Alberto Diaspro; Syed A.M. Tofail; Carmelina Ruggiero

doi:10.1371/journal.pone.0112192

Multilayered polyelectrolyte microcapsules: Interaction with the enzyme cytochrome c oxidase

Laura Pastorino
, Elena Dellacasa
, Mohamed R. Noor
, Tewfik Soulimane
, Paolo Bianchini
, Francesca D'Autilia
, Alexei Antipov
, Alberto Diaspro
, Syed A.M. Tofail
, Carmelina Ruggiero

University of Genoa
University of Limerick
Italian Institute of Technology
Plasmachem GmbH

Research output: Contribution to journal › Article › peer-review

Abstract

Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

Original language	English
Article number	e112192
Journal	PLoS ONE
Volume	9
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0112192
Publication status	Published - 5 Nov 2014

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abstract = "Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.",

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AU - Pastorino, Laura

AU - Dellacasa, Elena

AU - Noor, Mohamed R.

AU - Soulimane, Tewfik

AU - Bianchini, Paolo

AU - D'Autilia, Francesca

AU - Antipov, Alexei

AU - Diaspro, Alberto

AU - Tofail, Syed A.M.

AU - Ruggiero, Carmelina

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AB - Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

UR - https://www.scopus.com/pages/publications/84910642768

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AN - SCOPUS:84910642768

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VL - 9

JO - PLoS ONE

JF - PLoS ONE

IS - 11

M1 - e112192

ER -

Multilayered polyelectrolyte microcapsules: Interaction with the enzyme cytochrome c oxidase

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