Nanometer-scale physically adsorbed thermoresponsive films for cell culture

Deirdre Healy, Maria Nash, Alexander Gorelov, Kerry Thompson, Peter Dockery, Serguei Belochapkine, Julia Madden, Yury Rochev

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Physical adsorption was used to produce nanometer thick thermoresponsive films with a view to nonenzymatic cell detachment. Two polymers were investigated, poly-(N-isopropylacrylamide) and poly (N-isopropylacrylamide-co-N-tertbutylacrylamide). Substrates were prepared above and below the polymers’ LCST to investigate the effect of polymer conformation on the prepared substrates. Endothelial cells were seeded on the prepared films; cell proliferation was higher on the films produced below the polymers’ LCST than on those prepared above and cells detached from the surfaces upon temperature reduction. Physical adsorption of poly-(N-isopropylacrylamide)–based films is a viable approach to produce substrates compliant with cell growth and temperature modulated detachment.

    Original languageEnglish
    Pages (from-to)221-234
    Number of pages14
    JournalInternational Journal of Polymeric Materials and Polymeric Biomaterials
    Volume66
    Issue number5
    DOIs
    Publication statusPublished - 24 Mar 2017

    Keywords

    • Cell sheet detachment
    • physical adsorption
    • poly (N-isopropylacrylamide)
    • thermoresponsive polymers

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