TY - JOUR
T1 - Nanometer-scale physically adsorbed thermoresponsive films for cell culture
AU - Healy, Deirdre
AU - Nash, Maria
AU - Gorelov, Alexander
AU - Thompson, Kerry
AU - Dockery, Peter
AU - Belochapkine, Serguei
AU - Madden, Julia
AU - Rochev, Yury
N1 - Publisher Copyright:
© 2017, Copyright © Taylor & Francis.
PY - 2017/3/24
Y1 - 2017/3/24
N2 - Physical adsorption was used to produce nanometer thick thermoresponsive films with a view to nonenzymatic cell detachment. Two polymers were investigated, poly-(N-isopropylacrylamide) and poly (N-isopropylacrylamide-co-N-tertbutylacrylamide). Substrates were prepared above and below the polymers’ LCST to investigate the effect of polymer conformation on the prepared substrates. Endothelial cells were seeded on the prepared films; cell proliferation was higher on the films produced below the polymers’ LCST than on those prepared above and cells detached from the surfaces upon temperature reduction. Physical adsorption of poly-(N-isopropylacrylamide)–based films is a viable approach to produce substrates compliant with cell growth and temperature modulated detachment.
AB - Physical adsorption was used to produce nanometer thick thermoresponsive films with a view to nonenzymatic cell detachment. Two polymers were investigated, poly-(N-isopropylacrylamide) and poly (N-isopropylacrylamide-co-N-tertbutylacrylamide). Substrates were prepared above and below the polymers’ LCST to investigate the effect of polymer conformation on the prepared substrates. Endothelial cells were seeded on the prepared films; cell proliferation was higher on the films produced below the polymers’ LCST than on those prepared above and cells detached from the surfaces upon temperature reduction. Physical adsorption of poly-(N-isopropylacrylamide)–based films is a viable approach to produce substrates compliant with cell growth and temperature modulated detachment.
KW - Cell sheet detachment
KW - physical adsorption
KW - poly (N-isopropylacrylamide)
KW - thermoresponsive polymers
UR - http://www.scopus.com/inward/record.url?scp=84998679722&partnerID=8YFLogxK
U2 - 10.1080/00914037.2016.1201765
DO - 10.1080/00914037.2016.1201765
M3 - Article
AN - SCOPUS:84998679722
SN - 0091-4037
VL - 66
SP - 221
EP - 234
JO - International Journal of Polymeric Materials and Polymeric Biomaterials
JF - International Journal of Polymeric Materials and Polymeric Biomaterials
IS - 5
ER -