TY - JOUR
T1 - Nitrogen and sulfur co-doped carbon nanodots toward bovine hemoglobin
T2 - A fluorescence quenching mechanism investigation
AU - Wang, Jiajun
AU - Xiang, Xueqing
AU - Milcovich, Gesmi
AU - Chen, Jingru
AU - Chen, Chao
AU - Feng, Jiuju
AU - Hudson, Sarah P.
AU - Weng, Xuexiang
AU - Ruan, Yongming
N1 - Publisher Copyright:
© 2018 John Wiley & Sons, Ltd.
PY - 2019/2
Y1 - 2019/2
N2 - A deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co-doped carbon dots (N,S-CDs) with a quantum yield of 16% were synthesized by a 1-step hydrothermal method. The N,S-CDs exhibited a good dispersion, with a graphite-like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S-CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern-Volmer equation, temperature-dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S-CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S-CDs.
AB - A deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co-doped carbon dots (N,S-CDs) with a quantum yield of 16% were synthesized by a 1-step hydrothermal method. The N,S-CDs exhibited a good dispersion, with a graphite-like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S-CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern-Volmer equation, temperature-dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S-CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S-CDs.
KW - bovine hemoglobin
KW - fluorescence
KW - nitrogen and sulfur co-doped carbon dots
KW - quenching mechanism
UR - http://www.scopus.com/inward/record.url?scp=85052489302&partnerID=8YFLogxK
U2 - 10.1002/jmr.2761
DO - 10.1002/jmr.2761
M3 - Article
C2 - 30133028
AN - SCOPUS:85052489302
SN - 0952-3499
VL - 32
SP - -
JO - Journal of Molecular Recognition
JF - Journal of Molecular Recognition
IS - 2
M1 - e2761
ER -