NMR analysis of budding yeast metabolomics: A rapid method for sample preparation

C. Airoldi; F. Tripodi; C. Guzzi; R. Nicastro; P. Coccetti

doi:10.1039/c4mb00452c

NMR analysis of budding yeast metabolomics: A rapid method for sample preparation

C. Airoldi
, F. Tripodi
, C. Guzzi
, R. Nicastro
, P. Coccetti

University of Milan - Bicocca

Research output: Contribution to journal › Article › peer-review

Abstract

Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by ¹H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

Original language	English
Pages (from-to)	379-383
Number of pages	5
Journal	Molecular BioSystems
Volume	11
Issue number	2
DOIs	https://doi.org/10.1039/c4mb00452c
Publication status	Published - 1 Feb 2015
Externally published	Yes

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title = "NMR analysis of budding yeast metabolomics: A rapid method for sample preparation",

abstract = "Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.",

author = "C. Airoldi and F. Tripodi and C. Guzzi and R. Nicastro and P. Coccetti",

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T2 - A rapid method for sample preparation

AU - Airoldi, C.

AU - Tripodi, F.

AU - Guzzi, C.

AU - Nicastro, R.

AU - Coccetti, P.

N1 - Publisher Copyright: © The Royal Society of Chemistry 2015.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

AB - Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

UR - https://www.scopus.com/pages/publications/84921501176

U2 - 10.1039/c4mb00452c

DO - 10.1039/c4mb00452c

M3 - Article

C2 - 25333203

AN - SCOPUS:84921501176

SN - 1742-206X

VL - 11

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EP - 383

JO - Molecular BioSystems

JF - Molecular BioSystems

IS - 2

ER -

NMR analysis of budding yeast metabolomics: A rapid method for sample preparation

Abstract

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