Nonlinear opticalmicroscopy for drug delivery monitoring and cancer tissue imaging

Rabah Mouras; G. Rischitor; A. Downes; D. Salter; A. Elfick

doi:10.1002/jrs.2622

Nonlinear opticalmicroscopy for drug delivery monitoring and cancer tissue imaging

Rabah Mouras, G. Rischitor, A. Downes, D. Salter, A. Elfick

University of Edinburgh

Research output: Contribution to journal › Article › peer-review

Abstract

A multimodal nonlinear optical microscope that combines coherent anti-Stokes Raman scattering (CARS), two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and sum-frequency generation (SFG) was developed and applied to image breast cancer tissue and MCF-7 cells as well as monitoring anticancer drug delivery in live cells. TPEF imaging showed that drugs are preferentially localized in the cytoplasm and the nuclear envelope in resistant cells. Moreover, the extracellular matrix was observed by TPEF signals arising from elastin's autofluorescence and SHG signals from collagen fibrils in breast tissue sections. Additionally, CARS signals arising from proteins and (PO₂)^- allowed identification of tumors. Label-free imaging with chemical contrast of significant components of cancer cells and tissue suggests the potential of multimodal nonlinear optical microscopy for early detection and diagnosis of cancer.

Original language	English
Pages (from-to)	848-852
Number of pages	5
Journal	Journal of Raman Spectroscopy
Volume	41
Issue number	8
DOIs	https://doi.org/10.1002/jrs.2622
Publication status	Published - Aug 2010
Externally published	Yes

Keywords

Cars microscopy
Drug delivery
Live cell imaging
Nonlinear optical microscopy
Two photon fluorescence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jrs.2622

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title = "Nonlinear opticalmicroscopy for drug delivery monitoring and cancer tissue imaging",

abstract = "A multimodal nonlinear optical microscope that combines coherent anti-Stokes Raman scattering (CARS), two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and sum-frequency generation (SFG) was developed and applied to image breast cancer tissue and MCF-7 cells as well as monitoring anticancer drug delivery in live cells. TPEF imaging showed that drugs are preferentially localized in the cytoplasm and the nuclear envelope in resistant cells. Moreover, the extracellular matrix was observed by TPEF signals arising from elastin's autofluorescence and SHG signals from collagen fibrils in breast tissue sections. Additionally, CARS signals arising from proteins and (PO2)- allowed identification of tumors. Label-free imaging with chemical contrast of significant components of cancer cells and tissue suggests the potential of multimodal nonlinear optical microscopy for early detection and diagnosis of cancer.",

keywords = "Cars microscopy, Drug delivery, Live cell imaging, Nonlinear optical microscopy, Two photon fluorescence",

author = "Rabah Mouras and G. Rischitor and A. Downes and D. Salter and A. Elfick",

year = "2010",

month = aug,

doi = "10.1002/jrs.2622",

language = "English",

volume = "41",

pages = "848--852",

journal = "Journal of Raman Spectroscopy",

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TY - JOUR

T1 - Nonlinear opticalmicroscopy for drug delivery monitoring and cancer tissue imaging

AU - Mouras, Rabah

AU - Rischitor, G.

AU - Downes, A.

AU - Salter, D.

AU - Elfick, A.

PY - 2010/8

Y1 - 2010/8

N2 - A multimodal nonlinear optical microscope that combines coherent anti-Stokes Raman scattering (CARS), two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and sum-frequency generation (SFG) was developed and applied to image breast cancer tissue and MCF-7 cells as well as monitoring anticancer drug delivery in live cells. TPEF imaging showed that drugs are preferentially localized in the cytoplasm and the nuclear envelope in resistant cells. Moreover, the extracellular matrix was observed by TPEF signals arising from elastin's autofluorescence and SHG signals from collagen fibrils in breast tissue sections. Additionally, CARS signals arising from proteins and (PO2)- allowed identification of tumors. Label-free imaging with chemical contrast of significant components of cancer cells and tissue suggests the potential of multimodal nonlinear optical microscopy for early detection and diagnosis of cancer.

AB - A multimodal nonlinear optical microscope that combines coherent anti-Stokes Raman scattering (CARS), two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and sum-frequency generation (SFG) was developed and applied to image breast cancer tissue and MCF-7 cells as well as monitoring anticancer drug delivery in live cells. TPEF imaging showed that drugs are preferentially localized in the cytoplasm and the nuclear envelope in resistant cells. Moreover, the extracellular matrix was observed by TPEF signals arising from elastin's autofluorescence and SHG signals from collagen fibrils in breast tissue sections. Additionally, CARS signals arising from proteins and (PO2)- allowed identification of tumors. Label-free imaging with chemical contrast of significant components of cancer cells and tissue suggests the potential of multimodal nonlinear optical microscopy for early detection and diagnosis of cancer.

KW - Cars microscopy

KW - Drug delivery

KW - Live cell imaging

KW - Nonlinear optical microscopy

KW - Two photon fluorescence

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DO - 10.1002/jrs.2622

M3 - Article

AN - SCOPUS:77955499538

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JO - Journal of Raman Spectroscopy

JF - Journal of Raman Spectroscopy

IS - 8

ER -

Nonlinear opticalmicroscopy for drug delivery monitoring and cancer tissue imaging

Abstract

Keywords

UN SDGs

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