TY - JOUR
T1 - ns-µs time-resolved step-scan FTIR of ba3 oxidoreductase from Thermus thermophilus
T2 - Protonic connectivity of w941-w946-w927
AU - Nicolaides, Antonis
AU - Soulimane, Tewfik
AU - Varotsis, Constantinos
N1 - Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba3 oxidoreductase from Thermus thermophilus in the ns-µs time range and in the pH/pD 6-9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a3. Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a3 is described.
AB - Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba3 oxidoreductase from Thermus thermophilus in the ns-µs time range and in the pH/pD 6-9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a3. Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a3 is described.
KW - Cytochrome ba
KW - Heme-copper oxidoreductases
KW - Ns time-resolved step-scan FTIR
UR - http://www.scopus.com/inward/record.url?scp=84991396062&partnerID=8YFLogxK
U2 - 10.3390/ijms17101657
DO - 10.3390/ijms17101657
M3 - Article
AN - SCOPUS:84991396062
SN - 1661-6596
VL - 17
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 10
M1 - 1657
ER -