ns-µs time-resolved step-scan FTIR of ba3 oxidoreductase from Thermus thermophilus: Protonic connectivity of w941-w946-w927

Antonis Nicolaides; Tewfik Soulimane; Constantinos Varotsis

doi:10.3390/ijms17101657

ns-µs time-resolved step-scan FTIR of ba₃ oxidoreductase from Thermus thermophilus: Protonic connectivity of w941-w946-w927

Antonis Nicolaides
, Tewfik Soulimane
, Constantinos Varotsis

Cyprus University of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba₃ oxidoreductase from Thermus thermophilus in the ns-µs time range and in the pH/pD 6-9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a₃. Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a₃ is described.

Original language	English
Article number	1657
Journal	International Journal of Molecular Sciences
Volume	17
Issue number	10
DOIs	https://doi.org/10.3390/ijms17101657
Publication status	Published - 1 Oct 2016

Keywords

Cytochrome ba
Heme-copper oxidoreductases
Ns time-resolved step-scan FTIR

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10.3390/ijms17101657

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title = "ns-µs time-resolved step-scan FTIR of ba3 oxidoreductase from Thermus thermophilus: Protonic connectivity of w941-w946-w927",

abstract = "Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba3 oxidoreductase from Thermus thermophilus in the ns-µs time range and in the pH/pD 6-9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a3. Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a3 is described.",

keywords = "Cytochrome ba, Heme-copper oxidoreductases, Ns time-resolved step-scan FTIR",

author = "Antonis Nicolaides and Tewfik Soulimane and Constantinos Varotsis",

note = "Publisher Copyright: {\textcopyright} 2016 by the authors; licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/ijms17101657",

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T1 - ns-µs time-resolved step-scan FTIR of ba3 oxidoreductase from Thermus thermophilus

T2 - Protonic connectivity of w941-w946-w927

AU - Nicolaides, Antonis

AU - Soulimane, Tewfik

AU - Varotsis, Constantinos

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba3 oxidoreductase from Thermus thermophilus in the ns-µs time range and in the pH/pD 6-9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a3. Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a3 is described.

AB - Time-resolved step-scan FTIR spectroscopy has been employed to probe the dynamics of the ba3 oxidoreductase from Thermus thermophilus in the ns-µs time range and in the pH/pD 6-9 range. The data revealed a pH/pD sensitivity of the D372 residue and of the ring-A propionate of heme a3. Based on the observed transient changes a model in which the protonic connectivity of w941-w946-927 to the D372 and the ring-A propionate of heme a3 is described.

KW - Cytochrome ba

KW - Heme-copper oxidoreductases

KW - Ns time-resolved step-scan FTIR

UR - https://www.scopus.com/pages/publications/84991396062

U2 - 10.3390/ijms17101657

DO - 10.3390/ijms17101657

M3 - Article

AN - SCOPUS:84991396062

SN - 1661-6596

VL - 17

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 10

M1 - 1657

ER -

ns-µs time-resolved step-scan FTIR of ba₃ oxidoreductase from Thermus thermophilus: Protonic connectivity of w941-w946-w927

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Keywords

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