On the association between circulating biomarkers and atherosclerotic calcification in a cohort of arterial disease participants

Rachel M. Cahalane; Hilary E. Barrett; Aisling M. Ross; John J.E. Mulvihill; Helen Purtill; Logeswaran Selvarajah; Julie O'Brien; Eamon G. Kavanagh; Michael A. Moloneye; Siobhan M. Egan; Fiona C. Leahy; Tomás P. Griffin; M. N. Islam; Paul M. O'Shea; Michael T. Walsh; Eibhlís M. O'Connor

doi:10.1016/j.numecd.2021.02.005

On the association between circulating biomarkers and atherosclerotic calcification in a cohort of arterial disease participants

Rachel M. Cahalane, Hilary E. Barrett, Aisling M. Ross, John J.E. Mulvihill, Helen Purtill, Logeswaran Selvarajah, Julie O'Brien, Eamon G. Kavanagh, Michael A. Moloneye, Siobhan M. Egan, Fiona C. Leahy, Tomás P. Griffin, M. N. Islam, Paul M. O'Shea, Michael T. Walsh, Eibhlís M. O'Connor

Research output: Contribution to journal › Article › peer-review

Abstract

Background and aims: Atherosclerotic calcification is a powerful predictor of cardiovascular disease. This study aims to determine whether circulating levels of a local/systemic calcification inhibitor or a marker of bone formation correlate with measures of coronary or extracoronary calcification. Methods and results: Clinical computed tomography (CT) was performed on 64 arterial disease participants undergoing carotid and lower extremity endarterectomy. Coronary artery calcium (CAC) scores and volumes were acquired from the CT scans (n = 42). CAC scores and volumes were used to derive CAC density scores. Micro-CT was performed on excised carotid (n = 36) and lower extremity (n = 31) plaques to quantify the volume and volume fraction of extracoronary calcification. Circulating levels of dephospho-uncarboxylated Matrix Gla Protein (dp-ucMGP), fetuin-A, carboxylated and uncarboxylated osteocalcin (ucOC) were quantified using commercial immunoassays. Carotid participant CAC density scores were moderately negatively correlated with plasma dp-ucMGP (r_s = −0.592, P = 0.008). A weak negative association was found between CAC scores and %ucOC for all participants (r_s = −0.335, P = 0.040). Another weak negative correlation was observed between fetuin-A and the volume of calcification within excised carotid specimens (r_s = −0.366, P = 0.031). Despite substantial differences in coronary and extracoronary calcium measurements, the levels of circulating biomarkers did not vary significantly between carotid and lower extremity subgroups. Conclusion: Correlations identified between circulating biomarkers and measures of coronary and extracoronary calcium were not consistent among participant subgroups. Further research is required to determine the association between circulating biomarkers, coronary and extracoronary calcium.

Original language	English
Pages (from-to)	1533-1541
Number of pages	9
Journal	Nutrition, Metabolism and Cardiovascular Diseases
Volume	31
Issue number	5
DOIs	https://doi.org/10.1016/j.numecd.2021.02.005
Publication status	Published - 6 May 2021

Keywords

Cardiovascular disease
Computed tomography
Diagnostic testing
Fetuin-A
Matrix gla protein
Osteocalcin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.numecd.2021.02.005

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Cahalane, R. M., Barrett, H. E., Ross, A. M., Mulvihill, J. J. E., Purtill, H., Selvarajah, L., O'Brien, J., Kavanagh, E. G., Moloneye, M. A., Egan, S. M., Leahy, F. C., Griffin, T. P., Islam, M. N., O'Shea, P. M., Walsh, M. T., & O'Connor, E. M. (2021). On the association between circulating biomarkers and atherosclerotic calcification in a cohort of arterial disease participants. Nutrition, Metabolism and Cardiovascular Diseases, 31(5), 1533-1541. https://doi.org/10.1016/j.numecd.2021.02.005

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title = "On the association between circulating biomarkers and atherosclerotic calcification in a cohort of arterial disease participants",

abstract = "Background and aims: Atherosclerotic calcification is a powerful predictor of cardiovascular disease. This study aims to determine whether circulating levels of a local/systemic calcification inhibitor or a marker of bone formation correlate with measures of coronary or extracoronary calcification. Methods and results: Clinical computed tomography (CT) was performed on 64 arterial disease participants undergoing carotid and lower extremity endarterectomy. Coronary artery calcium (CAC) scores and volumes were acquired from the CT scans (n = 42). CAC scores and volumes were used to derive CAC density scores. Micro-CT was performed on excised carotid (n = 36) and lower extremity (n = 31) plaques to quantify the volume and volume fraction of extracoronary calcification. Circulating levels of dephospho-uncarboxylated Matrix Gla Protein (dp-ucMGP), fetuin-A, carboxylated and uncarboxylated osteocalcin (ucOC) were quantified using commercial immunoassays. Carotid participant CAC density scores were moderately negatively correlated with plasma dp-ucMGP (rs = −0.592, P = 0.008). A weak negative association was found between CAC scores and %ucOC for all participants (rs = −0.335, P = 0.040). Another weak negative correlation was observed between fetuin-A and the volume of calcification within excised carotid specimens (rs = −0.366, P = 0.031). Despite substantial differences in coronary and extracoronary calcium measurements, the levels of circulating biomarkers did not vary significantly between carotid and lower extremity subgroups. Conclusion: Correlations identified between circulating biomarkers and measures of coronary and extracoronary calcium were not consistent among participant subgroups. Further research is required to determine the association between circulating biomarkers, coronary and extracoronary calcium.",

keywords = "Cardiovascular disease, Computed tomography, Diagnostic testing, Fetuin-A, Matrix gla protein, Osteocalcin",

author = "Cahalane, {Rachel M.} and Barrett, {Hilary E.} and Ross, {Aisling M.} and Mulvihill, {John J.E.} and Helen Purtill and Logeswaran Selvarajah and Julie O'Brien and Kavanagh, {Eamon G.} and Moloneye, {Michael A.} and Egan, {Siobhan M.} and Leahy, {Fiona C.} and Griffin, {Tom{\'a}s P.} and Islam, {M. N.} and O'Shea, {Paul M.} and Walsh, {Michael T.} and O'Connor, {Eibhl{\'i}s M.}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Published by Elsevier B.V. on behalf of The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University",

year = "2021",

month = may,

day = "6",

doi = "10.1016/j.numecd.2021.02.005",

language = "English",

volume = "31",

pages = "1533--1541",

journal = "Nutrition, Metabolism and Cardiovascular Diseases",

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Cahalane, RM, Barrett, HE, Ross, AM , Mulvihill, JJE , Purtill, H, Selvarajah, L, O'Brien, J, Kavanagh, EG, Moloneye, MA, Egan, SM, Leahy, FC, Griffin, TP, Islam, MN, O'Shea, PM, Walsh, MT & O'Connor, EM 2021, 'On the association between circulating biomarkers and atherosclerotic calcification in a cohort of arterial disease participants', Nutrition, Metabolism and Cardiovascular Diseases, vol. 31, no. 5, pp. 1533-1541. https://doi.org/10.1016/j.numecd.2021.02.005

TY - JOUR

T1 - On the association between circulating biomarkers and atherosclerotic calcification in a cohort of arterial disease participants

AU - Cahalane, Rachel M.

AU - Barrett, Hilary E.

AU - Ross, Aisling M.

AU - Mulvihill, John J.E.

AU - Purtill, Helen

AU - Selvarajah, Logeswaran

AU - O'Brien, Julie

AU - Kavanagh, Eamon G.

AU - Moloneye, Michael A.

AU - Egan, Siobhan M.

AU - Leahy, Fiona C.

AU - Griffin, Tomás P.

AU - Islam, M. N.

AU - O'Shea, Paul M.

AU - Walsh, Michael T.

AU - O'Connor, Eibhlís M.

N1 - Publisher Copyright: © 2021 The Authors. Published by Elsevier B.V. on behalf of The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University

PY - 2021/5/6

Y1 - 2021/5/6

N2 - Background and aims: Atherosclerotic calcification is a powerful predictor of cardiovascular disease. This study aims to determine whether circulating levels of a local/systemic calcification inhibitor or a marker of bone formation correlate with measures of coronary or extracoronary calcification. Methods and results: Clinical computed tomography (CT) was performed on 64 arterial disease participants undergoing carotid and lower extremity endarterectomy. Coronary artery calcium (CAC) scores and volumes were acquired from the CT scans (n = 42). CAC scores and volumes were used to derive CAC density scores. Micro-CT was performed on excised carotid (n = 36) and lower extremity (n = 31) plaques to quantify the volume and volume fraction of extracoronary calcification. Circulating levels of dephospho-uncarboxylated Matrix Gla Protein (dp-ucMGP), fetuin-A, carboxylated and uncarboxylated osteocalcin (ucOC) were quantified using commercial immunoassays. Carotid participant CAC density scores were moderately negatively correlated with plasma dp-ucMGP (rs = −0.592, P = 0.008). A weak negative association was found between CAC scores and %ucOC for all participants (rs = −0.335, P = 0.040). Another weak negative correlation was observed between fetuin-A and the volume of calcification within excised carotid specimens (rs = −0.366, P = 0.031). Despite substantial differences in coronary and extracoronary calcium measurements, the levels of circulating biomarkers did not vary significantly between carotid and lower extremity subgroups. Conclusion: Correlations identified between circulating biomarkers and measures of coronary and extracoronary calcium were not consistent among participant subgroups. Further research is required to determine the association between circulating biomarkers, coronary and extracoronary calcium.

AB - Background and aims: Atherosclerotic calcification is a powerful predictor of cardiovascular disease. This study aims to determine whether circulating levels of a local/systemic calcification inhibitor or a marker of bone formation correlate with measures of coronary or extracoronary calcification. Methods and results: Clinical computed tomography (CT) was performed on 64 arterial disease participants undergoing carotid and lower extremity endarterectomy. Coronary artery calcium (CAC) scores and volumes were acquired from the CT scans (n = 42). CAC scores and volumes were used to derive CAC density scores. Micro-CT was performed on excised carotid (n = 36) and lower extremity (n = 31) plaques to quantify the volume and volume fraction of extracoronary calcification. Circulating levels of dephospho-uncarboxylated Matrix Gla Protein (dp-ucMGP), fetuin-A, carboxylated and uncarboxylated osteocalcin (ucOC) were quantified using commercial immunoassays. Carotid participant CAC density scores were moderately negatively correlated with plasma dp-ucMGP (rs = −0.592, P = 0.008). A weak negative association was found between CAC scores and %ucOC for all participants (rs = −0.335, P = 0.040). Another weak negative correlation was observed between fetuin-A and the volume of calcification within excised carotid specimens (rs = −0.366, P = 0.031). Despite substantial differences in coronary and extracoronary calcium measurements, the levels of circulating biomarkers did not vary significantly between carotid and lower extremity subgroups. Conclusion: Correlations identified between circulating biomarkers and measures of coronary and extracoronary calcium were not consistent among participant subgroups. Further research is required to determine the association between circulating biomarkers, coronary and extracoronary calcium.

KW - Cardiovascular disease

KW - Computed tomography

KW - Diagnostic testing

KW - Fetuin-A

KW - Matrix gla protein

KW - Osteocalcin

UR - http://www.scopus.com/inward/record.url?scp=85103477154&partnerID=8YFLogxK

U2 - 10.1016/j.numecd.2021.02.005

DO - 10.1016/j.numecd.2021.02.005

M3 - Article

C2 - 33810961

AN - SCOPUS:85103477154

SN - 0939-4753

VL - 31

SP - 1533

EP - 1541

JO - Nutrition, Metabolism and Cardiovascular Diseases

JF - Nutrition, Metabolism and Cardiovascular Diseases

IS - 5

ER -

On the association between circulating biomarkers and atherosclerotic calcification in a cohort of arterial disease participants

Abstract

Keywords

UN SDGs

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